基于稳定同位素标记的LC-MS方法及其在食品组学中的应用

当代食品科学研究已经从经典的方法向更全面的分析策略发展，其中，基于质谱的分析方法发挥着至关重要的作用。稳定同位素标记技术是近几年发展起来的广泛用于代谢组学的一种新技术,它克服了部分化合物内标获取困难的问题，可以实现对分析样品的绝对定量和相对定量分析。本项目拟通过合成一系列在电喷雾电离源条件下易电离，具有特征的串联质谱碎裂行为，能高效、选择性地与特定官能团反应的稳定同位素标记试剂，对食品中的化学成分进行标记；建立稳定同位素标记－双母离子/双中性丢失扫描方法，实现对食品中的化学成分进行非目标性的定性和定量分析，以解决食品组学中食品的质量、安全调控与可追溯性研究的问题。

Modern research in food science is moving from classical methodologies to comprehensive analytical strategies where mass spectrometry-based techniques play crucial roles. Stable isotope labeling strategy, which is widely used in metabolomics in recent years, has been developed to overcome the difficulty of obtaining internal standard compounds and achieve the goal of quantitative analysis. The current project aims to design and synthesize a series of stable isotope labelling reagents that can react with compounds containing specific functional group, and the labelled compounds can easily be ionized under electrospray ionization and generate characteristic fragments by tandem mass spectrometry. After optimizing of the labelling reaction conditions, we will develop a stable isotope labeling-double precursor ion/double neutral loss scan technique for the non-target qualitative and quantitative analysis of the chemical constituents in foods, which can largely benefit the foodomics research.