脂肪细胞分化过程包含大量的线粒体增殖和脂质沉积。过氧化物体增殖活化受体γ辅活化因子-1 alpha（Peroxisome Proliferator-activated Receptor γ Coactivator-1 alpha，PGC-1α）可以调控线粒体生物合成和氧化呼吸，直接影响脂质沉积，但其作用的分子机制尚不清楚。本项目拟以鸡腹脂原代培养的前脂肪细胞为材料，研究其分化过程中线粒体的发育性变化。同时采用超表达和基因干扰技术，分析PGC-1α基因对前脂肪细胞的线粒体发育、脂质沉积的影响。此外，基于本课题组对数字基因表达谱和生物信息学分析结果，进一步采用染色质免疫共沉淀和双重免疫荧光技术，研究PGC-1α活化过氧化物体增殖活化受体gamma（PPARγ）的分子机制。本项目拟通过上述研究探明PGC-1α通过PPARγ信号通路调控鸡前脂肪细胞分化的作用机制，为阐明鸡脂肪沉积机理提供新的思路。

A mass of mitochondrials proliferation and lipid acculumation are appeared in process of preadipocytes differentiation. Peroxisome Proliferator-activated Receptor γ Coactivator-1 alpha (PGC-1α) modulates mitochondrial biosynthesis and oxidation, which direactly affect lipid deposition in adipocytes. Until now, the molecular mechanism of PGC-1α controlling preadipocytes differentiation is not clear. Thus, in the present study, we will adopt primary preadipocytes from abdominal fat as materials and study on the developing process of mitochondrial. We are planning to analyze mitochondrial development, lipid deposition and cell differentiation via over-expression and siRNA of PGC-1α in preadipocytes. Furthermore, based on the data obtained in Digital Gene Expression and Bioinformation Analysis in previous study, we will adopt Chromatin Immunoprecipitation and Double Immunofluorescence Labeling methods to verify the mechanism of how PGC-1α activates its receptor Peroxisome Proliferator-activated Receptor γ (PPARγ). We will illuminate the molecular mechanism of PGC-1α regulating preadipocytes differentiation via PPARγ signaling pathway by putting these trials into practice. Our study would provide a new way to clarify the mechanism of fat deposition in chickens.