Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens.
Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens.
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Perilipin基因多态性可能影响中国肉用鸡的胴体性状
DOI：
10.5713/ajas.14.0554
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Zhang L;Zhu Q;Liu Y;Gilbert ER;Li D;Yin H;Wang Y;Yang Z;Wang Z;Yuan Y;Zhao X
在优质鸡育种项目中，肉质量的提高和肌肉产量的增加受到了极大的追捧。过去的研究表明，Perilipin基因(PLIN1)的多态性与哺乳动物的肥胖症高度相关，是改良鸡肉品质和屠体性状的潜在分子标记。在本研究中，我们用直接测序法对6个具有不同遗传背景的人群(共240人)进行了PLIN1基因所有外显子的单核苷酸多态(SNPs)筛查。我们评估了多态与屠体和肉质性状之间的关联。我们发现了位于10号染色体PLIN1基因5‘侧翼区和第1外显子的3个SNPs(分别为rs315831750、rs313726543和rs80724063)。根据这些SNPs构建了8种主要单倍型。计算了3个SNPs的等位基因频率、基因分型频率和遗传多样性参数。多态信息含量在0.2768~0.3750之间，反映了鸡的中等遗传多样性。CC、CT和TT基因在rs315831750上影响胸肌率(PBM)、腿肌率(PLM)和腹脂率(p<；0.05)。双倍型(单倍型对)影响全净膛重(PEW)和体质量(P<；0.05)。与携带其他二倍型的鸡相比，H3H7的PEW最大，H2的PBM最大，H7H7的PEW和PBM最小。因此，PLIN1基因多态性可能影响肉鸡的屠体和胸肌产量，H3H7和H2H2二倍型可能是提高肉鸡PEW和PBM的阳性分子标记。
Improved meat quality and greater muscle yield are highly sought after in high-quality chicken breeding programs. Past studies indicated that polymorphisms of the Perilipin gene (PLIN1) are highly associated with adiposity in mammals and are potential molecular markers for improving meat quality and carcass traits in chickens. In the present study, we screened single nucleotide polymorphisms (SNPs) in all exons of the PLIN1 gene with a direct sequencing method in six populations with different genetic backgrounds (total 240 individuals). We evaluated the association between the polymorphisms and carcass and meat quality traits. We identified three SNPs, located on the 5′ flanking region and exon 1 of PLIN1 on chromosome 10 (rs315831750, rs313726543, and rs80724063, respectively). Eight main haplotypes were constructed based on these SNPs. We calculated the allelic and genotypic frequencies, and genetic diversity parameters of the three SNPs. The polymorphism information content (PIC) ranged from 0.2768 to 0.3750, which reflected an intermediate genetic diversity for all chickens. The CC, CT, and TT genotypes influenced the percentage of breast muscle (PBM), percentage of leg muscle (PLM) and percentage of abdominal fat at rs315831750 (p<0.05). Diplotypes (haplotype pairs) affected the percentage of eviscerated weight (PEW) and PBM (p<0.05). Compared with chickens carrying other diplotypes, H3H7 had the greatest PEW and H2H2 had the greatest PBM, and those with diplotype H7H7 had the smallest PEW and PBM. We conclude that PLIN1 gene polymorphisms may affect broiler carcass and breast muscle yields, and diplotypes H3H7 and H2H2 could be positive molecular markers to enhance PEW and PBM in chickens.
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DOI：
10.5713/ajas.2013.13587
发表时间：
2014-08
期刊：
Asian-Australasian journal of animal sciences
影响因子：
--
作者：
Wang Y;Xiao LH;Zhao XL;Liu YP;Zhu Q
通讯作者：
Zhu Q
影响因子：
6.5
作者：
Kimmel AR;Brasaemle DL;McAndrews-Hill M;Sztalryd C;Londos C
通讯作者：
Londos C
影响因子：
5.6
作者：
Wang Y;Zhu Q;Yang L;Liu YP
通讯作者：
Liu YP
影响因子：
64.8
作者：
Rubin, Carl-Johan;Zody, Michael C.;Andersson, Leif
通讯作者：
Andersson, Leif
DOI：
10.1016/j.bbalip.2009.04.002
发表时间：
2009-06
影响因子：
4.8
作者：
Bickel, Perry E.;Tansey, John T.;Welte, Michael A.
通讯作者：
Welte, Michael A.