肥胖已成为严重威胁人类健康的十大疾病之一，如何有效减少和控制肥胖已成为当前面临的重要科学研究课题。从中药等天然产物中探寻减肥药物，具有西药无法比拟的安全性和有效性。本项目拟采用薄层色谱-生物自显影－实时直接分析－质谱联用技术，建立中药复杂体系中减肥活性成分的快速分离分析新策略。首先运用薄层色谱-生物自显影方法，实现色谱分离与活性检测的同步表征；进而运用原位、实时、直接（DART）-高分辨质谱分析，对目标成分进行快速鉴定；进一步运用LC-MSn进行特征离子捕获，建立反映特定中药内在品质的特征指纹图谱；再运用质谱导向的高效液相自动纯化系统，对目标成分进行高通量制备纯化并进行活性验证。该方法的建立与应用,一方面可快速发现减肥活性成分,为新型减肥药物研究提供先导化合物。另一方面,也为其他天然活性成分的快速筛选和制备提供思路和方法。

Obesity has become one of the top ten diseases that seriously threaten human health, and anti-obese or regulating body weight is a hot topic area of scientific research. Anti-obese drugs from TCM are considered as safer and more effective than Western medicine. The project is designed for a new strategy for interpretation of the anti-obese active components（lipase inhibitor） in Chinese medicines complex samples, by integrating Thin layer chromatography - Bioautography - Real-time direct analysis- Mass spectrometry- AutoPurification system（(TLC-Bioautography-DART-MS- AutoPurification）. As the first step，TLC-Bioautography will be conducted to indicating specific bioactivities of the spots in an in situ manner; Then the positive spots will be induced to a DART–MS for rapid identification of target constituents. A parallel sample of the same extractive of the crude drug will be detected by use of LC-MSn to display a characteristic fingerprinting profile．The intended fractions is subjected to the HPLC-MS-AutoPurification system for high-throughput preparation of the target compounds, which will be structurally identified and further assayed for bioactivities. This research will be benefit for rapid discovery of natural lipase inhibitors, providing lead compounds for new anti-obese drug research. Meanwhile, it will provide a new idea and new model for the rapid screening and separation of other natural active ingredients.