表面等离子共振microRNA快速高灵敏检测新技术的研究及其在胃癌诊疗中的应用

胃癌是我国最常见的恶性肿瘤之一, 严重地威胁着人民的生命，胃癌的早期诊断日益受到人们的关注。miRNA作为一种潜在的生物标志物，检测其表达水平对胃癌早期诊断、预后判断以及药物选择、疗效监测具有重要的意义。本项目针对现有检测分析方法费时费力、价格昂贵且灵敏度低等缺陷和不足，将表面等离子共振（SPR）、表面等离子共振成像（SPRi）技术与新型纳米材料、PCR、滚环扩增技术、亲和素-生物素系统、抗原抗体反应结合起来，利用高灵敏、高特异的生物识别分子，自组装生物功能化传感界面，原创性的提出一系列可以直接用于人体血液、组织、细胞等临床生物样本miRNA检测的超灵敏、高特异、高通量、实时快速的实用性检测新方法，从而为胃癌患者的早期诊断，分期，治疗和预后评估提供有力的依据。项目的实施对降低胃癌患者死亡率，保障人民健康和生命，促进国民经济发展具有重大意义。

Gastric cancer is one of the highest incidence malignant tumors in our country,threaten people's life seriously. The early diagnosis of gastric cancer is most concerned increasingly. As a new potential biomarker,miRNA detection has a great significance for the early diagnosis, judging severity, drug options, and therapeutic monitoring of gastric cancer. In order to overcome the drawbacks of the existing detection methods,which are time-consuming, laborious, expensive and poor sensitive, this project integrates the technology of surface plasmon resonance and surface plasmon resonance imaging with nano materials, PCR, rolling circle amplification, adivin-biotin system, and antibody reaction. Through the self-assembly biological function of sensing interface by using high sensitive and specific recognition molecules, a series of original technologys will be established and can be used for the rapid,ultrasensitive,specific,high throughput,realtime detection of miRNA from biological samples such as human body fluids, tissue, and cells.The fabricated platforms will provide new powerful tool for the early diagnosis, staging, treatment, and prediction of gastric cancer. The implementation of the project will have great significance to reduce the mortality, safeguard the people's health and life, and promote economic development.