从高等真菌中寻找高效低毒的新型抗肿瘤药物是当前研究热点。贺兰山生境特殊，孕育了丰富的高等真菌资源。本课题组前期研究发现，该地区的紫红丝膜菌、厚环粘盖牛肝菌和稀硬木层孔菌3种高等真菌提取物对五株人肿瘤细胞均表现了不同程度的抑制作用，其中石油醚和乙酸乙酯萃取部分是紫红丝膜菌的抗肿瘤活性部位，其石油醚和乙酸乙酯部位对白血病细胞（HL-60）的抗增殖作用最明显，IC50分别为10.56和17.21ug/mL。本项目拟继续采用活性跟踪分离方法，明确上述3种高等真菌的抗肿瘤活性成分，并通过结构改造制备衍生物探讨可能的构效关系，以及诱导细胞凋亡的生物活性研究探讨可能的作用机制，以期从中发现新颖的抗肿瘤先导化合物。

Searching the highly effective and lower toxic antitumor drugs from higher fungi has now become a hot topic studied by pharmaceutical researcher. For the geographic location,topographty and ecological environment, there are rich resources of higher fungi in Helan Mountain. We found the extracts of Cortinarius rufo-olivaceus, Suillus grevillei, and Phellinus robustas exhibited excellent cytotoxicities against five human tumor cell lines by MTS dye assay. The petrol ether and ethyl acetate extracts have significant cytotoxic actives against HL-60 with IC50 values of 10.56 and 17.21ug/mL, respectively. Based on the fact, this project, in combination with active tracking method, intends to get the novel leading compound of cytotoxic actives from three higher fungi through further separating, structural modifications and the possible mechanisms of the active compounds.