放疗后颌面部骨缺损修复由于骨组织再生能力差，修复效果不理想。恢复成骨和破骨功能是促进放疗区骨缺损修复的关键。课题组在前期研究中发现miR-34a正向调控辐照损伤后BMSCs的成骨分化，然而miR-34a是否参与调控辐照损伤后的破骨分化和成骨-破骨相互作用并不清楚。本项目拟在前期研究基础之上，通过体外细胞辐照模型，探讨辐照损伤对成骨和破骨前体细胞分化及相互作用的影响，研究miR-34a对辐照损伤后成骨和破骨功能的调控作用和机制，并通过动物模型验证miR-34a应用于放疗区骨缺损修复对骨再生的作用。本项目旨在阐明miR-34a对辐照后骨再生的调控作用，为利用miR-34a促进颌面部放疗区骨缺损修复提供实验依据。

Radiotherapy hampers bone regeneration and adds difficulties to the reconstruction of craniofacial defects. It is important to restore the function of osteogenesis and osteoclastogenesis to promote the repair of bone defects in the irradiated area. In the previous research, we found that miR-34a could enhance the osteoblastic differentiation of irradiated BMSCs. However, it is unclear whether miR-34a is involved in regulating osteoclast differentiation and osteoblast-osteoclast interaction. Therefore, based on previous research, this project intends to explore the influence of radiation on the differentiation and interaction of BMSCs and BMMs, and to study the effects of miR-34a on osteogenesis and osteoclastogenesis. Animal experiments will also be conducted to verify the function of miR-34a in bone regeneration after irradiation. This project aims to illuminate the effect of miR-34a on bone regeneration after irradiation, and provide experimental basis for targeting miR-34a as a therapeutic approach for enhancing the rehabilitation of irradiated bone defects.