ROS参与酿酒酵母高盐胁迫下细胞凋亡的机理研究

细胞凋亡，在生物体的生长发育、清除衰老和无用细胞维持细胞内环境稳定方面发挥重要作用，并且与人类的多种疾病密切相关。酿酒酵母是真核模式生物，且作为细胞凋亡模式生物的条件已初步成熟。高盐环境一直是人们关注的重要胁迫因子之一。项目申请人前期工作发现酿酒酵母线粒体DNA缺失菌株ρ0在高盐胁迫下发生细胞凋亡，并且该凋亡由细胞色素c介导。本项目是在申请者原有工作的基础上，拟从ρ0菌株高盐胁迫下活性氧ROS上升及细胞壁损伤方面，利用平板点滴生长测定分析、RNA-Seq分析、各种显微镜技术、蛋白分析等分子细胞生物学方法，初步探讨ρ0菌株高盐胁迫下ROS与细胞壁之间的关系，并进一步阐明参与ρ0菌株高盐胁迫下细胞凋亡的ROS与细胞壁相关基因及其作用机理和路径。为更深层次地解析高盐胁迫下酵母细胞凋亡分子机制奠定基础，从而对生命体的发育衰老及抗逆性有了更进一步的认识。

Apoptosis, is an intrinsic cell death process that plays a crucial role in developing and maintaining health by rapidly eliminating aging, useless or other potentially dangerous cells, and it is important for homeostasis and embryonic development. Also, apoptosis is closely related with many human diseases. Saccharomyces cerevisiae is the eukaryotic organism and now can be as the apoptosis model. Salt stress has always been one of the most important stress factors in the world. We previously found that yeast cells lacking mitochondrial DNA (ρ0) underwent apoptotic cell death induced by salt stress, and the apoptosis mediated by cytochrome c released from mitochondria to cytoplasm. Here, on the basis of our previous work and from the points of ROS accumulation and cell wall damage, We are using the molecular and cell biology technologies such as spot assay, RNA-Seq, microscopical techniques, protein analysis et al to discuss the relationship between ROS accumulation and cell wall damage in ρ0 cells under salt stress, and to further explore the mechanism of ROS and cell wall related genes involved in the apoptosis induced by salt stress in ρ0 cells. This research will lay the foundation for the further analysis of molecular mechanism of yeast cell apoptosis under salt stress, and from that we can get much more understandings in the aging and stress resistance of living organisms.