固有无序蛋白质在自然条件下呈“柔性”状态，不能独自形成稳定的三维结构，具有重要的生物学功能和独特的工作机制。疫霉菌产生大量效应子来调控植物的免疫反应，我们前期发现这些效应子中存在丰富的无序蛋白模块，其分布方式和保守性说明它们可能具有未知的重要功能。本研究拟选择大豆疫霉两个重要效应子（Avr1b和Avr1k）为对象，首先构建其N端转运区中的无序模块突变体，分析其对效应子转运和与磷脂酰肌醇结合的影响；然后构建C端功能域中无序模块突变体，分析其对诱导植物ETI和ETS发生的作用；最后结合酵母双杂交技术获得的Avr1b和Avr1k靶标蛋白，研究无序模块对其与靶标互作的影响及机制。本研究将明确这类无序模块在疫霉效应子中的功能与可能机制，在研究方法上为疫霉效应子研究提供新思路，在理论上有助于解释疫霉与寄主的分子互作基础。

Intrinsically disordered proteins (IDPs) are flexible proteins which lack a stable tertiary structrue under native conditions while possessing essential biological function and unique mechanism. Phytophthora sojae produces large number of effectors to manipulate plant immunity. We found abundant disordered protein modules with unusual distribution pattern and conservation in these effectors, suggesting potential important functions. Here, two P. sojae effectors, Avr1b and Avr1k, will be studied. The mutants of N-terminal disordered modules will be constructed to investigated the influence on the translocation and binding with phosphatidylinositol. The role of disordered modules in the process of inducing ETI and ETS will be studied by constructing C-terminal disordered modules. Combined with obtained target proteins of Avr1b and Avr1k by yeast two-hybrid system, we aim to explore the influence and mechanism of disordered modules in the interaction between effectors and targets. These results will aid the understanding of function and possible mechanism of disordered modules in Phytophthora effectors, and open new avenues to explore effector mechanisms, and provide important thenretical significance to explain the molecular basis of interaction between Phytophthora and plants.