胃癌是具有高度侵袭性并对生命有危害性的恶性肿瘤，上皮间质转化（EMT）是胃癌发生和转移过程中的重要事件。我们前期对90例胃癌病例mRNA及miRNA全基因组表达谱芯片进行整合分析，结果显示miRNA-200家族调控胃癌EMT，影响预后，其下游包括FRMD6，TEAD及LATS2等Hippo信号通路关键分子。本研究首先将在临床组织水平研究Hippo通路相关分子与胃癌EMT及预后的关联，随后采用分子和细胞生物学方法研究miR-200家族对FRMD6，TEAD及LATS2等靶向调控作用，以及改变Hippo信号通路后引发的EMT改变。最后将用体内动物实验证实部分研究结论。本研究将从机制上初步阐明miR-200家族通过靶向Hippo通路调控EMT的分子机制，为胃癌的分子机理研究和治疗靶标的筛选提供理论依据和实验基础。

Gastric cancer is a highly aggressive malignancy with high incidence and mortality worldwide. Epithelial-mesenchymal transition (EMT) is an important event in the occurrence and metastasis of gastric cancer. Our preliminary results of integrated analysis of microRNA and mRNA genome-wide expression profiles on 90 gastric cancer cases showed that the miRNA-200 family regulates EMT of gastric cancer and affects prognosis. In addition, its downstream targets include such as FRMD6, TEAD and LATS2. In this study, we will firstly focus on the association of Hippo-signaling pathway molecules with the EMT progress and prognosis of gastric cancer. Then we will study on the relationship between miR-200s and the predicted targets FRMD6, TEAD and LATS2. By using other molecular and cell biological methods, we will investigate the alteration of EMT after changing of hippo signaling pathway. Finally, some studies will conduct to validate the conclusions by animal model in vivo. Our preliminary study will elucidate the molecular mechanism of miR-200 family-Hippo pathway-EMT intermodulation mechanism, and provide a theoretical basis and experimental basis for the molecular mechanism of gastric cancer research and treatment.