番茄褪绿病毒（ToCV）是近年来在我国大面积发生的给番茄等蔬菜生产造成严重损失的病毒，前期研究发现Q烟粉虱的广泛分布是造成ToCV在最近几年迅速蔓延至全国的重要原因，然而关于影响Q烟粉虱获毒、持毒和传毒过程的关键因子仍缺乏研究，结合前期的转录组、基因组测序和qPCR验证结果，我们发现随着烟粉虱体内ToCV含量升高，α-葡糖苷酶基因表达量显著上升。本研究拟采用昆虫生理生化和分子生物学的技术与方法，重点研究α-葡糖苷酶在烟粉虱获毒、持毒、传毒过程中的作用机制，并通过酶抑制剂处理和RNAi的方法抑制α-葡糖苷酶的活性和基因表达情况，研究其活性降低后对烟粉虱获毒、持毒、传毒的影响及对烟粉虱生长发育和糖代谢的影响。预期研究结果将丰富我们对半持久病毒传播过程的认识，明确α-葡糖苷酶在烟粉虱传播ToCV过程中的作用机制，为有效控制ToCV提供新思路。

Tomato chlorosis virus (ToCV) has spread in China in recent years and causes serious losses to tomatoes and other vegetables. Previous studies found that the rapid spread of ToCV to the whole country in recent years is associated with the widespread distribution of Bemisia tabaci Q. However, little is known about the key factor that affects the process of virus acquisition, retention and transmission by B. tabaci Q. Combined with the previous transcriptome, genomic sequencing and qPCR results, we found that with the increase of ToCV content in B. tabaci, the expression of α-glucosidase gene increased significantly. This study intends to determine the effect of α-glucosidase on virus acquisition, retention and transmission by B. tabaci Q, using the techniques and methods of insect physiological biochemistry and molecular biology. After that, α-glucosidase activity and gene expression will be inhibited by enzyme inhibitor and RNAi treatment, then the inhibition effects on virus acquisition, retention and transmission by B. tabaci Q will be determined, and the inhibition effects on growth and development and glucose metabolism of B. tabaci Q will also be determined. Our expected results will help us to deeply understand the transmission process of semi-persistent virus, to determine the mechanism of α-glucosidase in ToCV transmission process by B. tabaci, and to provide new ideas for control ToCV effectively.