我们前期研制的单功能荧光量子点(PEG-QD-siRNA)突破性提高了siRNA的神经细胞转染率，抑制了β-分泌酶基因表达，为阿尔茨海默氏病(AD)治疗开拓了新途径，该论文即将在《自然出版集团》(Nature Publishing Group)杂志上发表(附件)。本课题拟合成新型多功能荧光量子点复合物(CUR/TAT-QD-siRNA)，该复合物在细胞膜穿透肽(TAT)引导下，携载姜黄素(CUR)和siRNA穿透血脑屏障，在病变脑区发挥CUR和siRNA联合对抗Aβ产生、聚集和形成老年斑，发挥预防和治疗AD的双重作用。另一特色是利用量子点(QD)的近红外光谱，可活体动物示踪该复合物的体内运输、穿透血脑屏障、脑靶向运输及药物代谢的全过程。项目预期目标是研制出携载CUR药物和siRNA基因药物且脑靶向运输的新型多功能荧光QD复合物，开发具有新机制、新结构的预防和治疗AD的特效药物。

Many researches focus on the functional quantum-dot-based siRNA delivery for cancer treatment, unlike the studies of the nerve disease treatment. In our previous studies, we developed the functionalized quantum dot compound (PEG-QD-siRNA) and obviously improved the transfection efficiency of siRNA in nerve cells. Furthermore, QD complexes restrained the expression of β-secretase (BACE1) gene for the treatment of Alzheimer's disease. We will synthesize a new-type multi-functional quantum dot compound in this study. In the cell membrane penetration peptide (TAT) guidance, the quantum dots carry the curcumin (CUR) and siRNA through the blood brain barrier and brain-targeted drug delivery. In lesion brain regions, CUR and siRNA restrain or inhibit the Aβ production, aggregation and senile plaque to play dual functions of the prevention and treatment of Alzheimer's disease. Use of the near infrared QD emission spectrum, we can trace the quantum dot compound including the transportation, penetration of the blood brain barrier and drug metabolism in living animals. Our paper related to previous study has been accepted by Nature Publishing Group (NPG). This project is expected to prepare a new-type multi-functional quantum dot compound that transport CUR and siRNA to brain. At last, we will develop a new way of the prevention and treatment of Alzheimer's disease.