本项目基于微泡与界面间距调控-微泡增强超声空化-细胞空蚀的思路，提出和研究制备血管内局部可控的易损斑块动物模型，并对斑块模型发展过程进行动态评价的方法，克服现有球囊损伤模型为圆周性损伤且大小、位置和程度不可控，与实际斑块差异显著等缺点。主要研究内容包括：1)在微流控细胞芯片上利用声光共聚焦的超高速显微和荧光成像方法研究流动条件下超声激励微泡对血管内皮细胞空蚀的瞬态过程及作用机制；2)利用全数字化超声成像平台，实现微泡与血管内膜间距的调控以及局部超声空化-空蚀所需的时间反转自适应聚焦，在活体兔腹主动脉内膜指定部位产生大小可控的损伤，结合高脂饮食诱发形成易损斑块；3)研究基于高帧率高分辨超声射频数据及图像的局部血管力学特性评价方法，结合血管组织病理学分析和血管内超声成像结果，确定局部血管力学特性变化与斑块组成及发展阶段之间的关系。可为血管易损斑块生理、病理和早期诊断成像研究提供新的有利工具。

In this project, based on microbubble enhanced ultrasound cavitation injury to vascular endothelial cell, the methods are proposed and studied to prepare a localized animal model of vulnerable plaque in a controlled manner and to evaluate the dynamic development process of the plaque model. The research is focused on: (1)With the microfluidic chips, the super high-speed microscopical photograph and fluorescence imaging are used to study the transient process and mechanism of the cavitation damage to vascular endothelial cell by ultrasound actived microbubble under shear flow conditions. (2)Using all-digital ultrasound imaging platform to regulate and control the distance between microbubbles and vascular intima, and make time reversed self-adaptive focus for localized ultrasound cavitation injure. After the injury with controlled size is generate at the specific site on rabbit abdomen aorta intima, vulnerable plaque can be induced with combined with high fat diets. (3) The method used to evaluate the mechanical properties of local vascular segment will be studied based on high frame rate and high resolution ultrasound RF data and imaging. Combined with the results of vascular histological analysis and intravascular ultrasound imaging, the relationship between the change of local vascular mechanical properties and the composition and development stage of plaque will be determined. This model could become a useful tool for the studies on pathologic, physiologic and early diagnostic imaging of vulnerable plaque.