本项目基于斑块的内皮损伤-反应理论，提出利用基于声势阱移动的超声操控方法调节动脉血管中高速流动的相变液滴与血管壁之间的距离，使其尽可能靠近管壁并进一步超声激励液滴相变、空化，对动脉血管内皮造成足以诱导形成动脉粥样硬化斑块的有效内皮损伤，并利用高分辨局部血管力学特性评价方法对损伤及模型发展过程进行活体评价，为易损斑块生理、病理和早期诊断成像研究提供有利工具。主要研究：1)利用声光同步检测研究微液滴声致相变的瞬态过程和声学响应；2)以椎骨作为反射面，通过频率阶梯改变移动超声驻波场中的声势阱，操控微液滴在管内聚集和移动的关键技术；3)构建高分辨超声图像引导的血管空化损伤活体动物实验系统，并发展相应的超声空化内皮损伤实验技术；4)在血管空化损伤和随后斑块形成过程中，利用高分辨局部血管力学特性评价方法结合血管组织病理学分析和血管内超声成像结果，确定局部血管力学特性变化与斑块组成及发展阶段之间的关系。

Based on the "Response to Injury Theory" of atherosclerosis, this project proposes a method for establishing the animal model of atherosclerosis plaque induced by controllable ultrasound cavitation arteriel endothelial injury combined with high fat diets.Effective ultrasound cavitation endothelial injury is realized by adjusting the distance between the phase-shift nanodroplets and the arterial vessel wall by manipulating the nanodroplets in high speed artery blood flow velocity with ultrasonic standing wave acoustic potential well shift, transporting the nanodroplets near to the desire site on the vessel wall, followed by acoustic droplet vaporization and cavitation under ultrasound exposure with higher ultrasound intensity to damage the vessel wall. And the mechanical properties of the local blood vessel with the cavitation endothelial injury are evaluated by using our hign resolution local vessel radial/circumferential strain imaging and super-short distance pulse wave velocity measurement method to in vivo evaluate the dynamic development process of the plaque. This model would become a potent tool for the studies on pathologic, physiologic and early diagnostic imaging of vulnerable plaque. The research is focused on: 1)the transient process of phase shifting and corresponding acoustic response of phase-shift droplets under focused ultrasound exposure with acoustic and optical method; 2)using rabbit vertebra as reflector, manipulate the phase-shift nanodroplets to be traped and shifted in the flowing vessel by shifting the acoustic potential well using ultrasonic standing wave with stepwise frequency; 3)built up a living animal experiment system for ultrasound image guided cavitation endothial damage, and develop the arterial cavitation injury process on this system; 4)during the process of plaque model establishement, combined with the results of vascular histological analysis and intravascular ultrasound imaging, the relationship between the change of local vascular mechanical properties and the composition and development stage of plaque will be determined.