禽腺病毒（FAdV）是一种呈世界性流行的dsDNA病毒，禽β防御素（AvBDs）在抗病毒免疫中发挥重要作用。新近研究发现，cGAS-STING是识别胞质dsDNA并启动天然免疫反应的重要感受通路，但FAdV激活cGAS-STING-AvBDs通路的调控机制尚不清楚。为此，在开展β防御素靶向禽肠上皮细胞调控作用的研究基础上，以FAdV-4感染鸡肝癌细胞株为研究对象，借助基因编辑、干扰和过表达及免疫共沉淀等技术，首先确定cGAS和STING与AvBDs的相关性；继而，开展FAdV-4诱导cGAS-STING对AvBDs的信号转导研究，筛选关键调控因子；通过FAdV-4与该通路的蛋白互作研究，确定作用靶点；最后，开展FAdV-4感染SPF鸡对cGAS-STING-AvBDs调控作用研究，进一步探明AvBDs抗病毒免疫调控机制。本项目为腺病毒有效防控提供可靠的理论依据具有重要科学意义。

Fowl adenovirus (FAdV) is a double-stranded DNA(ds DNA) virus which is distributed widely throughout the world, and avian β-defensins (AvBDs) play an important role in antiviral immunity. Recently, it has been found that cyclic GMP-AMP synthase- stimulator of interferon genes (cGAS-STING) is a sensory pathway that recognizes cytoplasmic dsDNA and initiates an innate immune response, but the regulatory mechanism by which FAdV activates the cGAS-STING-AvBDs pathway remains unclear. Therefore, based on the study on the regulatory effect of β-defensin targeting avian intestinal epithelial cells, FAdV serotype 4(FAdV-4) infected Chicken Liver Hepatocellular carcinoma cell line will be selected as research object, gene editing, over-expression and interference, co-immunoprecipitation and other techniques will be used to study the cGAS-STING-AvBDs pathway. Firstly, the correlation between cGAS-STING and AvBDs will be determined; then, the key regulatory factors in the signal transduction of FAdV-4-induced cGAS-STING to AvBDs will be screened;, and the target sites in protein interaction of FAdV-4 involved in the pathway will be determined; ; finally, the regulation of cGAS-STING-AvBDs by SPF chickens infected with FAdV-4 will be studied to explore the anti-viral immune regulation mechanism of AvBDs. This project will be of great scientific significance to provide reliable theoretical basis for the effective prevention and control of adenovirus.