化学感受蛋白CSP4在浆蜂哺育蜂感受幼虫信息素罗勒烯中的作用研究

浆蜂是我国从意大利蜜蜂中选育的蜂王浆高产品系，为我国蜂王浆产业贡献巨大。蜂群中，哺育蜂负责分泌蜂王浆哺育幼虫，这对幼虫发育、蜂群稳定及蜂王浆产业均具有重要意义。浆蜂蜂王浆高产与幼虫释放信息素诱发哺育蜂的哺育行为有关，但哺育蜂感受幼虫信息素机理尚不清楚。本人前期研究发现，浆蜂哺育蜂触角中化学感受蛋白CSP4增强表达，且在体外与幼虫信息素β-罗勒烯和别罗勒烯结合力很强，说明CSP4在哺育蜂哺育幼虫方面发挥重要作用。为进一步明确CSP4在浆蜂哺育蜂体内对两种罗勒烯的感受机制，本项目以浆蜂为对象，首先研究CSP4在哺育蜂触角和脑的免疫定位，明确其发挥作用的部位；然后利用RNAi抑制CSP4在哺育蜂体内表达，通过EAG和行为分析比较CSP4干涉的哺育蜂与对照组差异，最终确定CSP4在浆蜂哺育蜂中感受两种罗勒烯的作用。本项目在深入研究蜜蜂哺育行为中化学感受蛋白功能及幼虫信号传递机制方面具有重要意义。

Royal Jelly bee, selected from Italian bees (Apis mellifera) over four decades, is a high-yield royal jelly strain and makes great contributions to the royal jelly production in China.In the honeybee colonies, all brood care is performed by nurse bees that provide allparental care to their sister. The feeding behavior of nurse bees is of great significance to assisting the development of larvae, maintaining stable colony, and is thebasis of royal jelly industry.As we all known,high royal jelly yield is related to feeding behavior of nurse bees. But the mechanism of nurse bees identifying brood pheromone is still unclear. Previous study shows that chemosensory protein 4 (CSP4) is up-regulated in the royal jelly nurse bees and plays an important role in recognizing and binding larva pheromone β-ocimene and allo-ocimene in vitro. Those are benefit tostrengthen the feeding behavior. However, the distortion of CSP4 in the chemosensing system of Royal Jelly bee is unclear, and the mechanism of transporting β-ocimene and allo-ocimene in vivo is unknown. Hence, we will insight into the sense system of Royal Jelly bee and localize the expression of CSP4 in the antennae and brain of nurse bees with immunohistochemistry (IHC). Then, we send the synthetic RNA into the worker bees to control the CSP4 activity, and study the response of defectivebees to β-ocimene and allo-ocimene with EAG and behavioral observation. The project will help us to decipher the function of CSP4 and provide a basis for understanding the mechanism of CSP4 transporting brood pheromone β-ocimene and allo-ocimene.