抗体效应功能优化是抗体药物研发中的关键环节。包括噬菌体库技术在内的展示技术已经广泛的用于抗体体外进化。传统的抗体体外分子进化的方法往往具有突变随机、突变偏向、理论库容大、表位容易漂移等缺点。本课题拟基于计算机辅助平台建立"表位定向抗体库技术"，总结抗原-抗体作用模式与抗体效应（亲和力、稳定性等）间联系的一般规律，开展抗体的体外分子进化。表位定向抗体库能够针对抗体CDR区的关键位点设计理论优化的抗体突变，而且突变抗体识别的抗原表位都保持恒定。表位定向抗体库技术需要突变的位点少、理论库容小、表位不发生漂移，因此能够保持优化抗体的生物学活性，将为包括治疗性抗体在内的大分子药物的优化提供新策略。

To improve the biological efficacy of antibody is a key step in therapeutic antibody preparation. Conventionally, display technologies including phage library are widely exploited in molecular evolution of antibody in vitro. Traditional methods of in vitro evolution often have the shortage of random or deflection mutation, epitope variation, and requiring large library capacity. In this study, based on computer-aided platform, we will establish the technology of "Epitope-directed Library", and summarize the prevalent rules between the function mode of antigen-antibody complex and antibody effect, including affinity, stability, etc. for in vitro optimization of antibody. This technology enables a smaller content library with less mutated sites, and the epitope specificity can be retained to keep the biological function of antibody, thus it will be an improved strategy for optimization of large molecule drugs, e.g. therapeutic antibody.