质体分裂起始于FtsZ环，其定位是受Min系统调控。目前对于质体分裂与质体代谢物积累尚缺乏系统和整体的认知与理解。申请者在上一个基金资助下获得多个MeftsZs基因过量或抑制表达的田间表型差异显著的转基因株系，表明质体调控“牵一发动而全身”。我们提出假设：调控木薯质体分裂FtsZ环的形成和定位，影响质体分裂发生；质体反向信号与以糖代谢为中心的其他信号途径相互作用，影响质体内含物代谢及植株形态建成。本研究拟利用免疫荧光、木薯原生质体表达等手段，在细胞水平上观察不同FtsZ蛋白剂量下，MinD系统对FtsZ环调控策略；联合多组学探讨质体分裂影响质体代谢物积累的分子机制，解析转MeFtsZs基因株系的表型发生的调控网络。该项目的完成丰富了质体分裂影响植株代谢及形态建成的理论体系，为木薯种质创新提供了实践依据。

Plastid division begins in the FtsZ ring and its localization is regulated by the Min system. Current studies still could not elucidate the relations between plastid division and the accumulation of metabolites systematically and comprehensively. Under the previous fund, the applicant obtained a series of field transgenic lines with significant phenotypic differences in which the MeftsZs gene was overexpressed or inhibited, indicating that plastid regulation can affect the whole plant. We hypothesize that regulating the formation and localization of FtsZ-ring in cassava affects the occurrence of plastid division, and the plastid reverse signal interacts with other signal pathways centered on glucose metabolism to influence the accumulation of metabolites and morphogenesis of the whole plant. In this study, immunofluorescence and cassava protoplast expression system will be used to observe the regulation strategy of Min system on FtsZ-ring under different doses of FtsZ protein; an integrated analysis will be performed upon the transcriptome and proteome to probe into the molecular mechanism of plastid division affecting metabolite accumulation, and to reveal the regulatory network of MeFtsZs gene-mediated differential phenotypes. The completion of this project will enrich the theoretical system of plastid division affecting plant metabolism and morphogenesis, and provided a practical basis for cassava germplasm innovation.