水稻FINOR1基因调控重组频率的功能解析及应用研究

基因重组交换是图位克隆的核心，亦是作物遗传育种的关键科学问题。减数分裂过程中，重组交换的数目和分布受到严格调控，制约了作物新品种选育的进程。因此，科研工作者长期致力于解析重组交换调控的作用机理，找寻能提高重组交换的合理手段，应用于水稻育种实践。最新研究表明解旋酶FANCM的功能丧失能提高水稻重组频率，本项目拟以FANCM的互作因子FINOR1为切入点，通过细胞学、分子生物学、生物信息学等方法解析FINOR1基因的生物学功能；阐明FINOR1调控重组交换的遗传途径，并借助酵母双杂交、Co-IP、免疫荧光染色等实验进一步解析FINOR1蛋白与水稻重组交换蛋白的关系网；同时，通过构建finor1突变背景下的分离群体，明确finor1提高水稻杂交后代重组频率的效率，评价其育种潜力。通过上述研究，以期完善现有的减数分裂重组交换机制，并为实现水稻重组频率的遗传改良提供理论支持及基因资源。

Meiotic crossovers are at the heart of plant breeding and related genetic analyses such as gene mapping. In plants, the number and distribution of crossovers during meiosis is tightly constrained, limiting the efficiency of the breeding process and related activities. Therefore, researchers have been committed to analyzing the regulatory mechanism of meiotic crossovers, and looking for a reasonable means to improve recombination frequency in crop breeding. Recent studies have shown that the loss of function of DNA helicase FANCM can increase the recombination frequency in rice. In this proposal, we focus on a DNA binding protein, FINOR1, which is a FANCM interacting factor. We plan to explore the gene function and the genetic pathway of FINOR1 in modulating rice recombination frequency. Also, we intend to clarify the coordinated regulatory network involved in FINOR1, methods such as the yeast two-hybrid, Co-immunoprecipitation and immunofluorescence staining assays are all used in this proposal. At the same time, by constructing the isolated population in the background of finor1, we seek to explore the effect of finor1 on increasing the recombination frequency of rice hybrid progeny. The potential value of finor1 for crop breeding will also be verified. By these findings, it is expected to improve the existing meiotic recombination mechanism and provide theoretical support and genetic resources for increasing crop recombination frequency.