以发展蛋白质吸附理论和创新层析分离技术为目标，研究表面接枝聚合物配基吸附蛋白质过程的复杂性科学问题。首先构建以毛细管内表面蛋白质吸附和显微检测为主体的新型研究方法，以彻底消除传统方法中纳孔凝胶复杂结构对接枝链伸展、链间相互作用以及接枝链与蛋白质相互作用的影响，实现对蛋白质吸附-传质的微观定量；基于微观检测分析，建立毛细管内准确的蛋白质吸附平衡模型及其参数确定方法，在微观水平上定量分析接枝表面蛋白质吸附和分子传递；研究毛细管内表面接枝聚合物配基的蛋白质吸附-传质行为，揭示聚合物链结构（分支/线性）和配基结构（荷电性质和疏水性）等重要参数的影响规律；建立包括聚合物链结构、配基结构和表面密度等参数在内的接枝表面模型和蛋白质吸附-传递过程的多尺度模型，在分子水平上解析蛋白质吸附-传质行为和溶液环境的调控机制。研究成果对接枝聚合物配基理性设计和高效蛋白质层析技术发展具有重要理论意义。

Aiming at the theoretical development of protein adsorption and technological innovation of protein chromatography, this research will be devoted to the complex scientific issues of protein adsorption processes on polymeric ligand-grafted surfaces. First of all, a microscopic method to directly visualize protein adsorption and transport in bare capillaries modified with adsorptive ligands of proteins will be established, which should completely eliminate the effects of complex nanosized structures in hydrogel-based adsorbents used in traditional methods on polymer chain extension, interactions of polymer chains and the binding of protein to the chains, leading to the implementation of microscopic quantification of protein adsorption and transport. Based on the microscopic analysis, a method for reliable protein adsorption equilibrium model and its parameter determination procedure will be built up, which is the key to the microscopic quantification of protein adsorption and transport on polymeric ligand-grated surfaces. Then, various factors affecting protein adsorption and transport will be investigated in detail, including polymer chain structure (linear or branched), ligand structure (charge property and hydrophobicity) and so on. Finally, molecular models for the grafted surfaces (including polymer chain structure, ligand structure and surface density) and multiscale protein adsorption-transport models will be constructed to elucidate the molecular behavior of protein adsorption and transport on the grafted surfaces and the modulating mechanism of solvent environment. The research outcome will be of great significance in the rational design of polymeric ligands as well as the development of high-performance protein chromatography.