酿酒酵母的多组分底物共转运和共发酵是一个显著提高酵母发酵产乙醇效率的有效方法。蔗糖质原料特别是糖蜜原料中含有葡萄糖和蔗糖等原料，葡萄糖阻遏效应的存在影响了蔗糖原料的转运和利用。通过构建新的途径进行蔗糖的转运和细胞膜内水解将可以提高蔗糖的利用速度，提高蔗糖质原料的发酵效率。.本课题开展异源磷酸化分解系统在酵母中的整合研究，在酿酒酵母中导入蔗糖磷酸化酶，建立蔗糖在酵母胞内磷酸化分解的途径。开展蔗糖特异性跨膜转运系统和蔗糖的高亲和力研究，结合ITC技术和蛋白质分子改造技术对转运系统的蛋白质和底物的亲和力相关的关键氨基酸进行分析，提高蔗糖转运系统的转运效率和稳定性。通过蔗糖的代谢途径的重构，建立起一套适合酿酒酵母的蔗糖质原料的高效转化体系，从而为高效率的蔗糖质原料乙醇发酵建立研究基础。

The co-transporter and co-fermentation of multi-component substrates is an effective approach to significantly increase the production of ethanol in yeast. Sucrose feedstocks especial molasses contain glucose and sucrose, but the presence of glucose repression effects the transport of raw cane sugar and the use of sucrose.The utilization rate of the sucrose and the fermentation efficiency of sugar feedstock would be improved by building a new pathway to transport sucrose into the cell membrane and hydrolyze it..In this research, sucrose phosphorylation system be integrated into Saccharomyces cerevisiae cell would be carried out. The sucrose phosphorylase is introduced into Saccharomyces cerevisiae. Then, a phosphorylation and hydrolysis metabolic pathway of sucrose would be established in yeast.The high affinity research between the sucrose-specific transmembrane transport system and sucrose will be analyzed. Combined with ITC and protein modification technology, the key amino acids related to th affinity between the transport system protein and the substrate would be analyzed for enhancing the efficiency and the stability of transport systems. By reconstruction of sucrose metabolic pathway, an efficient sucrose conversion system suit for Saccharomyces cerevisiae would be builded. It is the basis for study efficient ethanol fermentation of sugar feedstocks.