碳量子点是一种10nm左右的新型纳米碳材料，兼具 良好的光致发光性能及生物与化学特性，是生物示踪成像等技术所期待的高性能荧光材料，非常引人瞩目。本项目旨在发展一条低成本的制备方法和新的性质调控方法。本项目通过分析生物质炭结构、特性及以往研究基础，提出了以廉价易得的生物质炭为原料的选择性氧化制备碳量子点方法，采用生物质炭原料结构调控碳量子点尺寸的思路，以及钝化是碳量子点表面基团与钝化剂之间反应的观点。研究以生物质炭、氧化剂和氧化条件为主要内容的选择性氧化体系，碳量子点发生团聚的条件，以及碳量子点表面化学结构和光致发光性质。通过综合分析，掌握生物质炭制备碳量子点的技术方法，生物质炭的类石墨微晶结构调控碳量子点尺寸，以及碳量子点的表面化学结构调控其光致发光性能等关键科学问题,促进碳量子点科学与技术的发展。在此基础上，研究生物质炭量子点负载DNA分子的表面化学结构，为开发其在转基因技术中的应用。

Carbon quautum dots are a new class of carbon nanomaterials with sizes below 10 nm.It has attracted wide attention because it posses a excellent photoluminescence ability and a appropriate properties in biology and chemistry , and thus show a great potenial in the field of biology and medical science as a desirous fluorescent material.This projext aim to develop a novel approach with a low cost to prepare carbon dots and to explore a new way to regulate its properties.This project put forward to a method of a selective oxidation to prepare carbon dots using the cheap and easily available biomass-based char as starting material, an approach to tune the size of carbon dots in terms of the structure of the starting material, and the viewpoint that surface passivation of carbon dots is in natue a chemical reaction between surface groups on carbon dots and possivation reagents. The project investigate the selective oxidation system including biomass-based char, oxidants, oxidation condition, the aggregation challenge of carbon nanoparticles in the process of seperation, and surface chemical structure and photoluminscene properties of the carbon dots. Based on the comprehensive analysis, one can mater the technological approach to prepare carbon dots from biomas-based chars, the way to regulat the size of carbon dots in terms of the size of like-graphite microcrystals in the char, and the way to gain the tunable photoluminscene properties in terms of surface chemical strutures of carbon dots, thus promoting the development of science and technology of carbon quantum dots. On the basis of these achievement, the surface chemical structure was required to support DNA molecular on carbon dots for the new application of carbon dot in the field of transgenic technology.