UV-C诱导葡萄果实黄烷醇类多酚和花色苷积累机制的研究

黄烷醇类多酚和花色苷是葡萄果实中主要的多酚类物质。前期研究表明，UV-C照射诱导葡萄果实黄烷醇类多酚和/或花色苷积累。但迄今为止，其诱导机理尚不明确。在前期研究基础上，本项目以红色品种赤霞珠（Cabernet Sauvignon）和白色品种霞多丽（Chardonnay）为试材，在果实发育过程中定期进行UV-C活体照射，以黄烷醇类多酚和花色苷生物合成关键酶DFR、LAR、ANS、ANR、UFGT为切入点，从底物、酶活性、酶蛋白含量及结构基因转录水平四个层次揭示UV-C诱导积累的机理；并就特异性调控花色苷合成的MybA1、MybA2和黄烷醇类多酚合成的MybPA1、MybPA2转录因子的表达特性进行研究，以期阐明UV-C诱导黄烷醇类多酚和花色苷积累的机制，特别是UV-C诱导黄烷醇类多酚和花色苷积累机制的差异，进一步完善紫外线诱导的多酚积累机理研究，为人为调控葡萄果实乃至葡萄酒品质提供理论依据。

Flavanols and anthocyanins are the main polyphenolic compounds in the grape berry. Numerous researches had idicated that the ultraviolet-C (UV-C) could induce the accumulation of flavanols and/or anthocyanins in the grape berry. However, the accumulation mechanisms of flavanols and anthocyanins induced by UV-C in grape berry were still obscure. Based on our previous researches, the grapevine (Vitis vinifera L. Cabernet Sauvignon and Chardonnay) were subjected to the regular UV-C irradiation during the berry development, and the temporal and spatial auucumlations of flavanols and anthoycanins in berries were studied fristly. Secondly, the expression of DFR, LAR, ANS, ANR, and UFGT, the key enzyme involved the biosynthesis of flavanols and anthoycanins in grape berry were analysed in terms of enzyme activity, protein amount, and mRNA transcriptional level, which should improve our understanding in the accumulation mechanism of flavanols and anthocyanins induced by UV-C in grape berry. Thirdly, the expression characteristics of MybA1, and MybA2, as well as MybPA1,and MybPA2, which regulated the biosynthesis anthocyanins and flavanols respectively in grape berry were also studied. This project was aimed to further illustrate the accumulation mechanism of flavanols and anthocyanins and flavanols in grape berry induced by UV-C, especially the differences in the accumulation mechanism of flavanols and anthocyanins, and provided a theoretical basis for the artificial regualtion of quality of grape berry and wine.