吸器是寄生植物连接寄主的特殊器官。本课题组前期研究发现，桑寄生种子虽然发芽率高，但寄生率很低，吸器是其种子能否成功寄生到寄主的关键。而吸器形成过程中其结构、内源激素、生理生化的变化及内部基因表达情况如何，是一个值得探讨的科学问题。故本研究拟围绕桑寄生种子萌发到吸器形成的整个发育阶段，进行显微与亚显微结构的观察及内源激素与生理生化等指标的测定，分析其形成的发育规律，明确桑寄生吸器形成的结构与生理机制；筛选吸器形成的关键时期，采用Solexa高通量测序技术进行转录组测序分析，挖掘调控桑寄生吸器形成过程中的关键基因，并进行RAEC克隆和RT-PCR验证。从结构、生理生化及分子水平上综合揭示桑寄生吸器的形成机理，为桑寄生乃至桑寄生科植物栽培技术的革新提供理论依据。

Abstract:Haustorium is a special organ of parasitic plant connect the host.Our previous studies showed that though the seed of Taxillus chinensis (DC.)Danser germination rate was high, the parasitic rate was very low, ha-ustorium is the key to the success parasitic seed to host. But in the process of the haustorium formation structure, endogenous hormones, internal physiological and biochemical changes,and how the internal gene expression,is a scientific problems worthy to be discussed.So this study by observing the microscopic and submicroscopic structure , determining the endogenous hormones and physiological and biochemical indexes from seed of Taxillus chinensis (DC.)Danser germination to the whole develop mental stages of haustorium formation，to analysis the formation rule of Taxillus chinensis (DC.)Danser ，and determine the critical period of its formation;The Solexa high-throughput sequencing technologies was used to analyze the transcriptome sequencing andexcavated the key genes that regulate the Taxillus chinensis (DC.)Danser haustoria formation process. and RAEC cloning and RT-PCR technologies also be used to verify the genes, which revealsed the haustorium formation mechanism from the molecular level, and provided a theoretical basis and technology innovation of the cultivation techniques for the Loranthaceae and Taxillus chinensis (DC.)Danser .