基质水分可控化是实现固态发酵规模化的关键技术。然而现有经验供液方式研究无法满足日益发展的固态发酵工业化需求，需要从原理上揭示供液调控机理，指导供液方式的固态发酵放大研究。本项目旨在基于构建的固态发酵有效供液技术，分别从基质、微生物及发酵体系三个层次深入解析基质有效供液促进麦秸固态发酵制备纤维素酶的作用机理。项目将建立基质物性参数表征体系，解析微生物在生理水平与分子水平上对供液调控的响应机制，并构建基于“分段拼接”方法的基质供液传质传热模型。本项目的实施将揭示基质供液促进固态发酵作用机理并实现其质热传递模型的理性构建，有效指导固态发酵供液方式放大研究，加快固态发酵工业化与可控化进程。

Achievement of controllable substrate moisture is crucial for large-scale solid-state fermentation (SSF). Regrettably, the existing empirical water supply methods could not support the modern SSF industry. Thus, it is crucial for theoretical study on the stimulation mechanisms of moisture controlling strategies before scaling up this technique. In this project, enhanced cellulase production by Trichoderma reesei on maize straw under solid-state fermentation is obtained by available water supply. The stimulation mechanisms will be analyzed from three levels of matrix, microbiology and fermentation system, respectively. Additional results include the followings: 1) The measure methods for matrix water characteristics, kinetics and thermodynamics parameters will be established from the view of physics; 2) The microbial response mechanism to available water supply will be explained at physiological and molecular levels; 3) An appropriate mass and heat transfer model for the water supply SSF will be constructed on the thought of “segmented mosaic”. This study will reveal the stimulation mechanisms of available water supply on SSF, achieve rational construction of mass and heat transfer model for SSF which will provide a guide to scaling up of water supply mode, and eventually accelerate the industrialization and controllability of modern SSF efficiently.