miRNA在临床上被视为新型生物标记物，为疾病早期和治疗术后提供全新的重要依据，具有深远而重大的研究意义。针对miRNA在体内含量极少、序列短、相似性高并容易被环境中RNA酶降解等难点，使得miRNAs的检测一直面临很多技术挑战。本项目采用微流控、光流控及液滴调控技术构建基于BRET原理检测液滴内miRNA的系统来实现高灵敏和高特异性识别。我们利用液滴微流控技术将供体、受体和miRNA液滴包裹，经过设计混合结构实现分子快速结合。电极诱导液滴注入微量底物为BRET提供能量。芯片中集成与液滴尺度匹配的光纤作为检测手段，结合微流控光学技术设计光学元件实现高效的荧光收集提升检测灵敏度。本项目融合微流控技术和微光电技术，通过新颖的芯片结构设计，独特的芯片集成技术，形成功能丰富的微全分析系统，为miRNA的高灵敏检测研究提供了一个全新高效的技术平台，为临床诊断和治疗提供高质量的检验依据。

miRNA has attracted extensive interest as a new biomarker, providing a novel and important evidence for the early stage of disease and post treatment. However, the detection of miRNAs has encountered many technical challenges, such as the low concentration level of miRNA in vivo, the short sequence, similarity and easy degradation by RNAase. In this project, A fiber integrated optofluidic system was.constructed to achieve high sensitivity and high specificity detection of miRNA using BRET principle. Firstly, donors, receptors and miRNA were encapsulated in droplets, and were mixed in channel to realize rapid bonding. Then the substrate was injected to the droplet by an electrode-induced method to facilitate enzyme catalysis and provide energy for BRET. We developed an innovative optofluidic platform that integrates the fiber and micro-optics for highly efficient fluorescence detection. This research combines the advantages of microfluidic and optofluidic technology, and provides an effective platform for the study of high sensitivity miRNA detection.