如何使肝星状细胞从活化状态恢复到正常静止态，抑制其大量分泌细胞外基质是肝纤维化治疗过程中面临的一个关键问题。为了探索其作用机制，本申请将对肝纤维化有抑制作用的纤连蛋白修饰到壳聚糖聚合物侧链上并形成超分子水凝胶，以此为模型，通过金属离子或pH的调控，对该超分子水凝胶的组装过程实现调控，在水凝胶发生溶胶-凝胶可逆转换的过程中，实现对材料力学性能和纤连蛋白释放的协同控制。在此基础上开展肝星状细胞粘附、生长、活化和凋亡等生理活动的研究，探索材料力学性能的变化和纤连蛋白的释放对肝星状细胞活化机制的影响规律，以及恢复到正常静止态的可行性，为肝纤维化的治疗尝试提供可行的研究模型和新的思路。

During the therapy of liver fibrosis, a key issue is how to recover actived hepatic stellate cells (HSC) to quiescent ones, and inhibit to secrete extracellular matrix (ECM). In order to explore its mechanism, in this project fibronectin that can inhibit liver fibrosis will be modified on the chitosan chains, and subsequently corresponding supramolecular hydrogel is construced and used as a model. The supramolecular assembling process is regulated by metal ions and pH value. During the transition of sol-gel, the synergy of mechanical property and fibronectin releasing is realized. Based on these results the researches on the biological behaviors of HSC (such as adhesion, growth, activation and apoptosis) are carried out. The rule of HSC activation is explored based on the changing of mechanical property and releasing of fibronectin. Moreover the possibility of recovering actived HSC to quiescent one is also evaluated. This project will try to offer possible research model and new insight for curing liver fibrosis.