乙型肝炎病毒（HBV）依然严重危害人类健康，挖掘开发高效低毒抗HBV药迫在眉睫。江南星蕨是苗族、仫佬族和土家族等多个少数民族的名药，调研发现它在民间还有治疗肝病之用。我们前期研究结果显示，江南星蕨提取物具有明显的抗HBV活性。在活性追踪指导下，该提取物用溶剂萃取后，经硅胶、凝胶和反相硅胶分别柱层析分离，我们从中得到了活性组分Fra.6I。经测定，Fra.6I在25 mg•L-1时，对HepG2.2.15细胞的HBsAg和HBeAg抑制率分别为98.2%和99.1%。这说明Fra.6I可能含有高活性抗HBV单体化合物。因此，本项目拟在此基础上进一步活性追踪分离，以明确其药效单体化合物，并通过结构修饰和体内、体外活性作用研究，初步明确其构效关系和可能作用机制，以期从江南星蕨发现新颖的抗HBV活性先导化合物，为HBV的有效防治提供新的途径。

Chronic hepatitis B (CHB) still severely does harm to human health, and screening nontoxic and effective anti-HBV drugs appears to be inevitable. Microsorium fortunei is a famous herbal of Miao, Mulao, Tujia, and other ethnic minorities. And investigation found that it is also used in the folk for the treatment of liver disease. Our pre-research showed that the extracts of Microsorium fortunei showed obvious anti-HBV activity. Guided by active tracking, an active fraction Fra.6I was obtained from the extracts by separating through solvent extraction, and chromatography with silica, sephadex LH-20 and ODS, respectively. And the inhibition ratio of Fra.6I (25 mg•L-1) to HBSAg and HBeAg of HepG2.2.15 cells could reach up to 98.2% and 99.1%, respectively. From this result, we can see clearly that the fraction Fra.6I may contain highly active anti-HBV compounds. Based on the fact, this project, in combination with active tracking method, intends to get the novel leading compound of anti-HBV activity from Microsorium fortunei through further separating, structural modifications and research of the possible mechanisms of the active compounds in vitro and in vivo experiments. The results may provide a new approach for the effective prevention and control of HBV.