玫瑰黄链霉菌（Streptomyces roseoflavus）Men-myco-93-63是本课题组筛选得到并研究多年的一株生防链霉菌，近年来我们发现该菌株可产生多烯大环内酯类抗生素，其主要成分为roflamycoin，对灰霉病菌、黄萎病菌等十多种重要植物病原菌具有良好的抑制作用，EC50值可达1.82-12.18μg/ml，具有作为农用抗生素的良好潜力，但目前尚未有其生物合成途径和在植保领域研究应用的报道。在前期研究基础上，本项目将对生防菌Men-myco-93-63进行全基因组测序和生物信息学分析，探究其抑菌活性代谢产物roflamycoin的抗生素生物合成途径，寻找和确定该抗生素合成基因簇中关键的生物合成基因及途径特异性调控基因，通过接合转移方法对关键基因进行敲除和回补从而验证其功能，为提高抗生素产量和获得新的多烯大环内酯类化合物提供重要的依据，促进我国新农用抗生素的研发和应用。

Streptomyces roseoflavus Men-myco-93-63 is a biocontrol Streptomyces strain screened and studied by our group for many years. In recent years, we have found that it can produce a group of polyene macrolide antibiotics, whose main component is roflamycoin having good inhibition effect on more than ten important plant pathogenic fungi, such as Botrytis cinerea and Verticillium wilt, and the EC50 value of it is from 1.82 to 12.18 μg/ml. This antibiotic has good potential as an agricultural antibiotic, however, there are no reports on its biosynthetic pathway and application in plant protection. On the basis of previous studies, genome-wide sequencing of Men-myco-93-63 and bioinformatics analysis will be developed in this project, the biosynthetic pathway of antimicrobial metabolite roflamycoin will be explored, and the key biosynthetic genes or pathway-specific regulatory genes in the antibiotic gene cluster will be analyzed. The key genes will be knocked out and replenished by conjugation transfer method to verify their functions, which will provide an important basis for improving antibiotic production or obtain new polyene macrolides, and promote the development and application of new agricultural antibiotics.