ROS和NO对沉水植物穿叶眼子菜通气组织形成过程的调控机制

穿叶眼子菜是眼子菜科眼子菜属一种多年生沉水植物，由于长期适应水下的低氧环境，穿叶眼子菜根茎叶中演化出发达的通气组织。目前已知水生植物通气组织的形成是低氧引发信号网络调控细胞程序性死亡(PCD)的结果，但有关ROS和NO在错综复杂的信号网络中相互作用调控PCD的发生机制尚不清楚。本研究以沉水植物穿叶眼子菜为材料，采用结构植物学、细胞学和分子生物学等技术，研究ROS和NO对穿叶眼子菜通气组织PCD特征的影响；利用生理生态学和药理学方法，研究穿叶眼子菜ROS和NO的合成与代谢及对通气组织的调控作用，揭示ROS和NO在通气组织形成过程中的生理调控机制，并基于转录组测序和qRT-PCR技术，分析与活性氧产生相关的RBOH基因，以及与NO合成相关的NOS和NR基因在通气组织形成过程中的表达特征，从基因水平探讨ROS和NO对通气组织形成的分子调控机制，为提高植物对缺氧的适应性提供新思路和理论依据。

Potamogeton perfoliatus，belongs to Potamogeton in Potamogetonaceae, is a perennial submerged plant. Due to its long-term adaptation to the low-oxygen environment under water, P. perfoliatus has developed rich aerenchyma in its vegetative organ. Currently, it is known that the formation of aerenchyma in aquatic plants is the result of the regulation of programmed cell death (PCD) by the signaling network induced by hypoxia, but the mechanism of the interaction between ROS and NO in the intricate signaling network to regulate PCD remains unclear. In this study, P. perfoliatus was employed as the experiment materials. The effects of ROS and NO on the PCD characteristics during aerenchyma formation process were studied using structural botany, cytology, molecular biology techniques. The synthesis and metabolism of ROS and NO and its regulatory role on aerenchyma formation in P. perfoliatus were studied by physiological and pharmacological methods, to reveal the physiological mechanism of ROS and NO on the aerenchyma formation. And on the base of the transcriptome sequencing and qRT-PCR technology, the expression of RBOH genes associated with ROS synthesis, NOS and NR gene associated with NO synthesis were investigated in the process of aerenchyma formation. The aim is to explore ROS and NO molecular regulation mechanism of aerenchyma formation from the level of gene expression, and to provide new ideas and theoretical basis for improving the adaptability of plants to hypoxia.