联合应用生物支架材料负载脂肪干细胞移植促进脊髓神经再生机制研究

脊髓损伤（SCI）后的神经再生和功能修复是世界性难题，其关键性原因是影响再生原因众多，缺乏对其再生修复机制全面了解。项目组前期分别应用生物支架材料或干细胞移植研究发现均能在不同程度促进SCI后神经联系重建和功能恢复。为此本课题提出：联合应用生物支架材料负载脂肪干细胞移植能否通过改善脊髓局部微环境、激活宿主神经干细胞增殖和分化、促进轴突再生、重建神经纤维联系等机制实现感觉和运动功能的恢复？本项目拟构建大鼠完全性SCI模型，对联合治疗方案的功效、安全性和机制进行比较研究，并对其机制等方面予以验证。为SCI的临床试验研究和SCI患者治疗提供理论依据和新的策略。

Nerve regeneration and functional recovery after spinal cord injury (SCI) are worldwide problems. Many factors affect neural regeneration, but the regeneration mechanism has not been comprehensively understood. Our previous studies found that biological materials or cell transplantation promoted neural reconstruction and functional recovery after SCI. Therefore, the current study propose that the recovery of sensory and motor functions can be achieved by improving the local microenvironment of the spinal cord by biological scaffold materials combining with adipose-derived stem cells. The endogenous neural stem cells can also be activated and further proliferate and differentiate into neurons, reconstructing connections with regeneratiing axons. We intend to establish an animal model of acute complete SCI in rats to compare the efficacy, safety of the combined therapy. And We're going to explore the mechanism. The proposed study in this project will provide theory and new strategies for clinical trial research and treatment of SCI patients.