Human Adipose-Derived Stem Cells Combined with Nano-Hydrogel Promote Functional Recovery after Spinal Cord Injury in Rats.
Human Adipose-Derived Stem Cells Combined with Nano-Hydrogel Promote Functional Recovery after Spinal Cord Injury in Rats.
复制标题
人脂肪干细胞复合纳米水凝胶促进大鼠脊髓损伤后功能恢复
DOI：
10.3390/biology11050781
复制
发表时间：
2022-05-20
期刊：
影响因子：
4.2
通讯作者：
Wu, Wutian
中科院分区：
文献类型：
--
作者：
Li, Jianping;Ji, Zhisheng;Wang, Yu;Li, Tiantian;Luo, Jinghua;Li, Jun;Shi, Xueshuang;Li, Liming;He, Liumin;Wu, Wutian
脊髓损伤后的神经再生和功能恢复是世界性难题。科学家们在使用天然或合成材料修复脊髓损伤方面取得了令人鼓舞的成果。本文报道纳米水凝胶联合人脂肪干细胞调节炎症微环境，保护神经元和轴突，促进运动功能恢复。此外，通过液相色谱-质谱联用技术筛选出三个与神经元和轴突生长相关的蛋白质。这些结果为脊髓损伤的临床治疗提供了依据。脊髓损伤的治疗旨在重建纤维连接，恢复中断的神经通路。脂肪间充质干细胞（ADSCs）可以促进脊髓损伤后运动功能的恢复。然而，脂肪干细胞的存活率低和局部移植后损伤部位外的渗漏减少了细胞的数量，这严重削弱了累积效应。我们使用自组装水凝胶RADA 16-RGD（R：精氨酸; A：丙氨酸; D：天冬氨酸; G：甘氨酸）内装载的人ADSC对严重脊髓损伤的大鼠进行异位移植。我们的结果表明，人ADSCs与RADA 16-RGD的联合移植改善了损伤部位ADSCs的存活。炎性反应受到抑制，神经元存活率提高，神经纤维和髓鞘蛋白残留面积增加。通过Basso、Beattie和Bresnahan（BBB）运动评定量表评分和电生理测量确定，功能行为得到促进。ADSCs可促进脊髓损伤的修复。本研究为脊髓损伤的治疗提供了新的思路。
Nerve regeneration and functional recovery after spinal cord injury (SCI) are worldwide problems. Scientists have achieved encouraging results in the repair of spinal cord injuries using natural or synthetic materials. In this paper, we report that nano-hydrogel combined with human adipose-derived stem cells regulate the inflammatory microenvironment, protect neurons and axons, and promote motor function recovery. In addition, three proteins related to neuronal and axonal growth were screened by Liquid chromatography-mass spectrometry. These results provide evidence for clinical treatment of spinal cord injury. The treatment of spinal cord injury aims to reconstruct the fiber connection and restore the interrupted neural pathways. Adipose mesenchymal stem cells (ADSCs) can promote the recovery of motor functions in spinal cord injury. However, poor survival of ADSCs and leakage outside of the injury site after local transplantation reduce the number of cells, which seriously attenuates the cumulative effect. We performed heterotopic transplantation on rats with severe spinal cord injury using human ADSCs loaded within self-assembly hydrogel RADA16-RGD (R: arginine; A: alanine; D: aspartic acid; G: glycine). Our results indicate that the combined transplantation of human ADSCs with RADA16-RGD improved the survival of ADSCs at the injured site. The inflammatory reaction was inhibited, with improved survival of the neurons and increased residual area of nerve fibers and myelin protein. The functional behaviors were promoted, as determined by the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale score and electrophysiological measurements. ADSCs can promote the repair of spinal cord injury. This study provides new ideas for the treatment of spinal cord injury.
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影响因子：
2.5
作者：
CUEVAS, P;CARCELLER, F;GIMENEZGALLEGO, G
通讯作者：
GIMENEZGALLEGO, G
DOI：
10.1073/pnas.97.12.6728
发表时间：
2000-06-06
影响因子：
11.1
作者：
Holmes, TC;de Lacalle, S;Zhang, SG
通讯作者：
Zhang, SG
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影响因子：
6.1
作者：
Ji ZS;Li JP;Fu CH;Luo JX;Yang H;Zhang GW;Wu W;Lin HS
通讯作者：
Lin HS
影响因子：
4.3
作者：
Gao L;Peng Y;Xu W;He P;Li T;Lu X;Chen G
通讯作者：
Chen G
影响因子：
5.6
作者：
Kilroy, Gail E.;Foster, Sandra J.;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.