CXCL12/CXCR4/CXCR7在受精过程中的作用及分子机制

近年来我国不孕症发病率逐年上升，既影响男女双方生殖健康，还产生相关社会问题。尽管生殖医学中心胚胎培养技术不断改进，仍无法避免受精失败的发生。我们前期研究发现CXCL12/CXCR4/CXCR7是介导母-胎界面各种细胞相互作用的关键分子；近期研究发现人成熟卵母细胞表达CXCL12与CXCR4，而人精子表达其受体CXCR4与CXCR7，提示CXCL12/CXCR4/CXCR7不仅有助于母-胎界面形成，还是介导精子与卵母细胞相互作用的关键分子。本课题拟解析CXCL12/CXCR4/CXCR7在人卵母细胞及精子的表达规律；比较它们在正常和受精失败患者卵母细胞及精子的表达模式；通过体外实验和动物模型解析 CXCL12/CXCR4/CXCR7介导卵母细胞与精子生物学相互作用的分子机制。研究结果将有助于揭示受精的分子机制，对阐述不孕症的发病机理，临床受精失败的治疗及实现ART技术安全应用均有积极意义。

Fertilization failure is still inevitable in ART treatment despite of the advancement in embryo culture technology. Our previous researches indicate the role of CXCL12/CXCR4/CXCR7 in the interaction of fetal-derived trophoblasts and materno-derived cells at the materno-fetal interface. The recent study also confirms the existence of CXCL12/CXCR4 in human mature oocytes and CXCR4/CXCR7 in human spermtozoa, respectively. The results suggest that CXCL12/CXCR4/CXCR7 axis is involved in mediating oocyte-sperm cooperation and fertilization. Thus, in the present study, we plan to compare the expression pattern of CXCL12/CXCR4/CXCR7 in human oocytes and spermtozoa between successful fertilization and fertilization failure patients. The modulation of CXCL12/CXCR4/CXCR7 on oocyte-sperm interaction is investigated via the in vitro cell culture system and in vivo animal model. The purpose of this study is to elucidate the role of CXCL12/CXCR4/CXCR7 axis in fertilization, which contributes to understanding the mechanisms of infertility and is beneficial to clinical diagnosis and treatment.