很多蛋白质是一些癌症和传染性疾病的标志物，而它们的浓度在疾病发展的早期阶段非常低。因此在后基因组时代，建立和发展蛋白质的超高灵敏的检测方法显得极为重要。 本项目拟将荧光探针Ru-dppz配合物类发光探针用于检测PCR产物，并将这些探针用于免疫-PCR分析，提高免疫-PCR技术的灵敏度。探索各种基于三螺旋DNA 形成原理的双螺旋DNA 序列识别分析新方法，并将新方法用于PCR产物检测；进一步与免疫-PCR技术相结合，建立基于三螺旋DNA的免疫-PCR新分析方法。将新建立的免疫-PCR 分析方法推广用于不同疾病抗原的检测，发展和建立不同疾病抗原的高灵敏检测方法。

Many proteins are biomarker of some cancer and contagious disease， but their concentration is very low at the early period of disease. Therefore, it is of importance to develop supper-sensitive method?for dection of protein on the postgenome era.? The project will detect PCR product by using Ru-dppz complexes as luminescent probe, and the luminescent probe will be further used in immuno-PCR to improve its sensitivity. Different new methods for sequence-specific recognition of dsDNA will be developed based upon triplex formation, then applied it to detect PCR product, further combined with immnuo-PCR, to establish novel immuno-PCR sensor based upon the formation of triplex DNA. The new immuno-PCR sensors will be extended to detect the antigen of different diseases, to develop new methods for ultrasensitive dection of antigen for different diseases.