iASPP是新近发现的一个p53的重要拮抗基因，在白血病细胞高表达，且可抑制白血病细胞凋亡。我们前期通过酵母双杂交发现iASPP与Sertad1结合。本项目针对iASPP与Sertad1相互作用在急性白血病增殖和凋亡中的作用进行研究：1、明确Sertad1蛋白能够与iASPP结合。2、确定Sertad1与iASPP相互作用的结构域。3、探讨iASPP3与Sertad1结合对两者表达和稳定的影响。4、研究iASPP与Sertad11结合对p53促转录活性的影响。5、探讨iASPP与Sertad1结合对白血病细胞周期、凋亡、增殖的影响。本项目会有助于进一步阐明白血病细胞的增殖、凋亡的调控机制，发现白血病新的治疗靶点。

The iASPP as a novel suprressor of P53 is overexpressed and may inhibit apoptosis. Through yeast two-hybridization, we have demonstrated that iASPP binds Sertad1.In this proposal, we will investigate the roles of interaction between iASPP and Sertad1 in proliferation and apoptosis of leukemia cells.First,the interaction between iASPP and Sertad1 will be further verified.Second,we will identify the domain of Sertad1 mediated for binding iASPP.Third,the effect of interaction on expression and stability of iASPP and Sertad1 interaction will be illustrated.Forth, we will explore the impact of iASPP and Sertad1 interaction on transactivation mediated by p53. Fifth，the impact on cell cycle,apoptosis and proliferation brought by interaction between iASPP and Sertad1 will be investigated. This project may contribute to further reveal regulation mechanism of proliferation and apoptosis in leukemia cells, and identify novel targets for leukemia therapy.