水稻SSIIIa-Dnak蛋白互作及其对籽粒抗性淀粉形成的代谢调控

抗性淀粉对糖尿病、结肠癌等膳食相关的慢性病具有很好的预防和控制作用。水稻SSIIIa基因是调控抗性淀粉性状的关键基因，但受waxy等其它因子影响。SSIIIa主要以蛋白复合体形式调控淀粉代谢，活力缺失会导致蛋白复合体形成模式的改变。本项目以遗传背景相同的ssIIIa等位突变体及其亲本为材料，在对籽粒转录组和相关基因表达量进行分析的基础上，筛选鉴定与SSIIIa互作的蛋白，并对前期筛选获得的Dnak蛋白进行互作验证和功能研究，探究SSIIIa-Dnak互作对淀粉合成的调控及脂肪代谢的可能影响，初步阐明基于SSIIIa的淀粉-脂肪平衡调控，以进一步完善SSIIIa为核心的抗性淀粉分子调控网络，挖掘抗性淀粉合成新基因，推动高抗性淀粉等功能水稻育种。

Resistant starch shows good prevention and controlling for the diabetes, colon cancer and etc. SSIIIa was the major gene for resistant starch, but the regulation was dependent on the genotype of waxy. Usually, SSIIIa functions in starch synthesis as protein complexes, the loss of SSIIIa may change the modes of protein complexes. In this project, according to the transcriptomes of allelic ssIIIa mutants and their parent, and the expression levels of some target genes, we try to obtained some new proteins interacted with SSIIIa, and further confirm the interactions between SSIIIa and Dnak which were identified in preliminary study. Furthermore, the functions of Dnak and the regulation mechanisms of SSIIIa-Dnak interaction on resistant starch metabolite and possible influences on lipids metabolite will be explored. That will helpful to elucidate the balance regulation between starch and lipid metabolite via SSIIIa, improve furtherly the regulation network of resistant starch via SSIIIa. The complement of this project might also promote digging out novel genes related to resistant starch and functional rice breeding, i.e. high resistant starch rice breeding.