稻米非淀粉多糖-淀粉的互作及其消化和品质效应

非淀粉多糖主要以纤维素、半纤维素和果胶等物质存在于细胞壁中。籽粒胚乳中非淀粉多糖对谷物的品质影响巨大。但由于自然界中水稻非淀粉多糖含量仅0.3%-0.7%，且水稻主要以精米饭为食用方式，鲜见对水稻内源非淀粉多糖的相关研究。本项目以高非淀粉多糖含量的不同来源等位胚乳突变体cw及国内外核种质为材料，对非淀粉多糖的主要成份、含量及稻米的消化、蒸煮食味品质相关理化特性进行分析，研究非淀粉多糖-淀粉复合体的形成及其对稻米的消化特性和蒸煮食味品质的影响，明确非淀粉多糖-淀粉的互作机理及其缓消化机理和对稻米的品质效应；同时，利用胚乳细胞突变体对籽粒的糖代谢谱进行分析，阐明非淀粉多糖对稻米品质效应可能的代谢调控，开发非淀粉多糖分析的实用检测标记，构建优质稻米的辅助选择体系，为改良稻米蒸煮食味品质提供新的研究思路，也可为水稻功能成份育种提供理论支撑。

Non-starch polysaccharides (NSPs) exist in cell wall as cellulose, hellicellulose, pectin and etc, which play important effects on the quality of grains. While there were very few studies on NSPs in rice as for the content of NSPs was only 0.3%-0.7% and also rice was eaten as cooked rice. In this project, endosperm mutants cw with high NSPs derived form different background and some rice core germplasm were selected to analyze the properties of NSPs including structure, constituents, content and the digestion, cooking and eating quality of rice, to study the formation of NSPs-starch complex and its’ influences on the properties of digestion and quality, which might help to explicit the mechanism of NSPs-starch interactions, it influences on rice digestion and quality; What’s more, the mutants and its’ parent were selected to analyze the metabolite profiling of sugars fractions to explore the possible metabolite regulation on rice quality by NSPs. Markers for detecting NSPs will be developed to construct a platform for selecting rice with good or special quality. That will pave a new way for improving rice quality, and also can provide some theoretic supports for functional breeding.