本研究以不吸水链霉菌为材料，通过基因交叉回补、RT-PCR、EMSA、启动子修改等方法，研究不吸水链霉菌中PAS-LuxR家族调控子在四霉素和制霉菌素生物合成中的独立调控问题，以阐明PAS-LuxR家族调控子精确调控机制。研究内容包括：1、PAS-LuxR家族调控子浓度对其独立调控的影响；2、天然的调控子和结合序列在体外结合能力；3、TtmRIV中PAS区域的氨基端25~43位氨基酸较其它调控子多的20个氨基酸残基对结合的影响；4、确定结合位点上-5/4位和-7/6位碱基对结合效果的影响；5、TtmRIV对改造启动子后的基因的调控情况。PAS-LuxR家族调控子在不吸水链霉菌中独立调控机制的明确，有利于对多烯大环内酯类抗生素生物合成中的调控更深入地认识，并且为此类抗生素利用精确调控定向育种奠定理论基础。

In this study, the independent regulation of PAS-LuxR family regulators in the biosynthesis of tetramycin and nystatin in Streptomyces ahygroscopicus was studied by means of gene cross-complementation, RT-PCR, EMSA and promoter modification to clarify the precise regulation mechanism of PAS-LuxR family regulators. Research contents include: the effect of the concentration of PAS-LuxR family regulators on their independent regulation; binding ability of natural regulators and binding sequences in vivo; the effect of 20 more amino acid residues at amino terminus 25-43 position of the PAS region of TtmRIV on the binding level; the influence of the -5th and 4th position bases and the -7th and 6th position bases on the binding effect; regulation level of TtmRIV on the these genes after the modification of promoter. The clarification of regulation mechanism of PAS-LuxR family will lead deeper understanding of regulation in biosynthesis of polyene macrolide, and lay the foundation for directional breeding by accurate regulation.