Abl和trx基因影响家蚕茧丝产量的作用机制研究

茧丝性状是决定蚕桑行业产量和质量效益的主要因素，充分了解和研究家蚕茧丝性状，对选育茧丝性状优良的蚕品种、提高蚕茧生产效益有着重要的意义。项目组前期的研究显示，Abl和trx基因在丝腺细胞增殖和丝蛋白合成过程中具有重要调控作用。本研究利用CRISPR/Cas9基因组编辑技术、家蚕转基因技术、高通量测序和生物信息学分析等方法，深入研究Abl和trx基因在家蚕茧丝性状中的调控作用机制。该研究为利用转基因与基因组编辑技术辅助传统遗传育种方法选育高产、优质的家蚕品种打下基础，对家蚕多丝量品种的选育有着重要借鉴意义，并对家蚕丝腺生物反应器的研发具有指导作用。

Silk properties are the main factors that determine the yield and quality benefits of the sericulture industry. It is of great significance to fully understand and study the cocoon and silk properties of the silkworm to select the silkworm varieties with good cocoon and silk properties and improve the cocoon production benefits. Our previous studies have shown that the Abl and Trx genes play an important role in the regulation of silk gland cell proliferation and silk protein synthesis. In this study, CRISPR / cas9 genome editing technology, silkworm transgenic technology, high-throughput sequencing and bioinformatics analysis were used to study the regulatory mechanism of Abl and trx genes in the properties of cocoon silk. This study will lay a foundation for the selection of high-yield and high-quality silkworm varieties by using transgenic technology and genome editing technology assisted with traditional genetic breeding technology. It will be of great significance to the selection of silkworm varieties with multi silk content, and will be of guiding significance to the research and development of silkworm silk gland bioreactor.