茧丝是蚕桑生产的主要目标产品，茧丝产量是家蚕品种中最受重视的性状之一。本课题在前期茧丝产量性状QTL定位的工作基础上，拟运用转录组测序技术构建家蚕多丝量品种和少丝量品种及其近等基因系的差异基因表达谱，筛选获得茧丝产量的关键功能基因，分析这些基因的表达特征、差异剪接、转录调控机制，并采用转基因过表达及RNAi技术进行基因功能验证，从而解析影响家蚕产量性状的分子机理。为从分子水平上阐明家蚕茧丝产量性状差异形成的机制、以及为家蚕丝腺生物反应器的研发提供借鉴。

Cocoon and silk production quantities are one the most important characters in sericulture.Functional genes of silk production remain to be discovered, which would be valuable if used in the future silkworm breeding. In this research, we will use high-throughput RNA sequencing to analyze the transcriptome of several silkworm strains with different silk productivities, and based on the previous QTL mapped regions, to screen out the differentially expressed genes in different strains. Then，the expression patterns, transcript regulation and potential alternative splicing of these genes, and as well as the protein interactions will be explored with multiple techniques. The gene functions will be further verified in transgenetic silkworm and by RNA interference (RNAi) technique. With this research, we aim to find important genes related to cocoon and silk production characters, and to analyze the regulatory mechanism of these genes on silk production. This research will lay important foundation for improving cocoon silk yield and the silkgland bio-reactor.