家蚕对AcMNPV的感染机制研究

核型多角体病毒病是蚕业生产中危害最严重的病害之一。已有的研究显示，家蚕对苜蓿银纹夜蛾核型多角体病毒（AcMNPV）的感染性由隐性单基因(susceptible to AcMNPV,sav) 控制。在已经对该基因精细定位的基础上，分析定位区间的所有18个预测基因，比较这些基因序列在感染型和不感染型亲本及近等基因系间的差异，克隆出差异的基因并进行功能分析与验证，从而解析相关基因的作用机制；采用转录组技术，筛选宿主中与病毒侵染、复制与增殖相关的关键基因，研究该杆状病毒与家蚕宿主之间的互作关系，探讨杆状病毒在家蚕体内侵染、复制的过程以及家蚕对杆状病毒抗性的分子机制，为家蚕抗核型多角体病毒病育种提供理论基础。

Nuclear polyhedrosis virus is one of the most harmful disease in sericulture. The former research showed that the susceptiblity to Autographa californica nuclear polyhedrosis virus （AcMNPV）(sav) was controlled by one recessive gene. Based on these results of fine mapping sav. After that, the 18 predicted genes located in the mapping region will be cloned and analyzed. The genes which are different expressed in the parents (length and quantity) and near isogenic lines will be analyzed and rescued by transgenic silkworms methods. The important gene which engaged in the infection, duplication of AcMNPV in Bombyx mori will be analyzed by transcriptome methods.The molecular mechanism of the virus infection and duplication in the silkworm will be discussed based on the results of map-based cloning and yeast-two-hybrids.This research will provide a theoretical basis for breeding silkworm strains resistant to BmNPV.