脂质分子重构制备内酯类风味化合物的酶学基础研究

内酯类风味化合物是重要的食品添加剂之一，市场需求巨大。生物制造内酯类风味化合物是未来发展趋势，而“酶的发现与应用”是其核心竞争点。只有解决了“酶芯片”问题，才能解除被“卡脖子”的现状。基于此，本项目拟开展“脂质分子重构制备内酯类风味化合物的酶学基础研究”，以内酯类风味物质的酶法合成为目标导向，建立基于脂质分子酶法重构的不同合成体系；根据真实反应过程需要，采取组学与传统方法相结合的新酶发现与筛选新技术获取可实现催化的酶分子；并采用结构生物学、生物计算、酶学和分子生物学等技术手段，深入理解酶的结构与功能关系，获得催化性能良好的酶分子，同时揭示酶催化理论；建立和优化不同酶的组合高效催化体系并揭示其过程调控新机制。通过本研究，可推动酶工程领域的基础学科发展，丰富酶学理论，并获得具有应用前景的酶制剂；推动内酯类风味化合物的酶法催化转化新技术发展，同时为多酶催化体系的建立奠定理论基础。

Lactones are one kind of the important flavor compounds, and its market demand is huge. Bio-production of lactones can be considered as a more promising strategy in future, thus discovery and application of enzymes seem to be fatal for the commercial competition. To solve the problem of "enzyme chip", can help to overcome the current situation of "stuck neck". Therefore, this project intends to carry out the basic enzymatic research on the preparation of lactones via lipid molecule reconstruction, aiming at obtaining lactones by enzymatic synthesis, and different synthetic systems were to establish based on the enzymatic reconstruction of lipid molecule. According to the needs of real reaction process, combination of new enzymatic discovery, screening technologies based on genomic mining and traditional methods are conducted to obtain novel catalystes. By means of structural biology, biological calculation, enzymology and molecular biology, we can deeply understand the structure-function relationship of enzymes, obtain enzymatic molecules with good catalytic performance, reveal the theory of enzymatic catalysis, establish and optimize the efficient catalytic system of different enzymes and reveal the new mechanism of their process regulation. Through this study, we can promote the development of basic disciplines in the field of enzyme engineering, enrich the theory of enzymology, and obtain enzymatic preparations with promising application prospects. Our work can also promote the development of new enzymatic catalytic transformation technologies for lactones production, and lay a theoretical foundation for the establishment of multi-enzyme catalytic system.