成纤维细胞生长因子受体2（FGFR2）在ASCs修复尿道缺损中作用的实验研究

长段尿道狭窄和缺损因缺乏合适的替代材料是泌尿外科的棘手问题。组织工程学给我们提供了新的方法和思路。脂肪干细胞（ASCs）是具有临床应用潜力的种子细胞，前期研究提示其可同支架材料修复尿道缺损，但其在体内分化的机制并不明确。成纤维生长因子及其受体（FGF/FGFR）调控广泛的细胞功能，包括细胞的增殖和分化。FGFR2被认为是尿路上皮成熟的关键因素。因此我们推测ASCs表达FGFR2通过FGF通路调节其在体内向尿路上皮的分化。本研究利用慢病毒过表达或敲低FGFR2在ASCs的表达水平，观察相应ASCs在体内的分化情况，证实FGFR2在ASCs分化中起到重要作用，为促进尿路修复寻找新的靶点。

Long urethral stricture has bothered urologists for years due to the lack of optimal substiute tissue. Tissue engineering shows us a new way to treat the disease. Adipose stem cells（ASCs）have the potential of clinical application. ASCs seeded scaffolds show good results in urethra reconstruction. But it is still unknown that how did ASCs differentiate in vivo.Fibroblast growth factor and fibroblast growth factor receptor (FGF/FGFR) mediate a broad range of cell funcitons,including proliferation and differentiation. FGFR2 plays an important role in the maturation of urothelium.So we presume that ASCs differentiate into urothelial cells in vivo by FGFR2 through FGF signalling.In this study, to tesify the important role of FGFR2 in ASCs differentiation, we use lentivirus to knock down or overexpress the FGFR2 expression level in ASCs and analyse its differentiation status in vivo.