基于miRNA-9对下丘脑室旁核小胶质细胞P2X7受体的调节探讨电针对SHR大鼠交感神经兴奋性的影响

针刺降压机制研究一直是高血压防治的关注热点。本课题组在以往承担的国自然课题研究中发现，针刺“太冲”可以影响下丘脑相关基因及蛋白的表达。为进一步阐明针刺“太冲”降压的中枢效应机制，本研究选用自发性高血压（SHR）大鼠为模型，运用Western Blot、ELISA、免疫组化、脑立体定位核团灌注等技术，以期从miRNA-9介导下丘脑室旁核小胶质细胞P2X7受体表达，影响PI3K/AKT/NF-κB通路的角度，探讨电针“太冲”对SHR大鼠交感神经兴奋性调控的中枢机制，进一步阐明电针“太冲”的降压作用。同时为高血压防治提供新的靶点和科学依据。

Study on the mechanism of acupuncture to reduce blood pressure It has always been the focus of hypertension prevention and treatment。In the previous research of national nature, our group found that acupuncture "Taichong" can affect the expression of related gene and protein in hypothalamus.In order to further clarify the central mechanism of acupuncture "Taichong" in reducing blood pressure, SHR rats were selected as the model,Western blot, ELISA, immunohistochemistry and brain stereotactic perfusion were used,In order to explore the central mechanism of electroacupuncture "Taichong" on the regulation of sympathetic nerve excitability in SHR rats, and further clarify the antihypertensive effect of electroacupuncture "Taichong" from the perspective of mirna-9 mediated P2X7 receptor expression in the microglia in paraventricular nucleus of hypothalamus and the effect of PI3K / Akt / NF - κB pathway. At the same time, it provides a new target and scientific basis for the prevention and treatment of hypertension.