针刺补泻手法长期以来都是国内外针灸界争议的焦点之一。本课题组在此前承担的国家自然基金课题研究中发现捻转补泻手法具有不同的生物学效应，并可以通过血管内皮因子、下丘脑基因和蛋白表达、血管内皮细胞信号转导通路和神经放电等机制调控血压。为进一步探讨捻转补泻手法的效应机制，本研究以自发性高血压大鼠为研究对象，利用PET脑功能成像技术，QT-PCR技术及Western-blot技术，阐明捻转补泻手法的中枢整合机制，构建捻转补泻手法调控血压的信号传导网络，并筛选出网络中的关键基因和蛋白，以期阐明捻转补泻手法的作用机制，同时为高血压的防治提供可靠的理论支持和技术方法。

Manipulation of twirling reinforcing-reducing of Acupuncture has been a controversial focus of Acupuncture and moxibustion cirle at home and abroad. This task group has found that the manipulation of twirling reinforcing-reducing has different biological effects and can regulating blood pressure by mechanisms of vascular endothelial growth factor, gene and protein expression in hypothalamus , vascular endothelial cell signal transduction pathways and neuronal discharge in the former National Natural Science Foundation of China. For further study on the effect mechanism of the manipulation of twirling reinforcing-reducing, this research select the spontaneously hypertensive rats as the research object, PET brain imaging technology ，RT-PCR technology and Western-blot technology as well be used to clarify the central integration mechanism of the manipulation of twirling reinforcing-reducing and construct the signal transduction networks of this manipulation on blood pressure regulation to clarify the effect mechanism of the manipulation of twirling reinforcing-reducing and provide a reliable theoretical support and technical method. for the prevention and treatment of hypertension.