虎皮病是梨贮运过程中极易发生的一种严重的生理病害，显著影响了果实的外观品质，造成巨大的经济损失。研究发现，抗氧化酶谷胱苷肽-S-转移酶（GST）可能通过影响活性氧（ROS）代谢参与虎皮病的发病过程，但尚缺乏实验验证。本项目以‘砀山酥梨’为试材，利用iTRAQ技术比较不同病情等级梨果皮蛋白谱的差异，结合生物信息学分析、蛋白印迹和干预性试验，筛选出与发病过程密切相关的关键GST基因；将其构建到超表达载体pCAMBIA1301上转化番茄，通过比较野生型和转基因后代果实在低温胁迫下的表型差异，探明其在ROS代谢中的作用；最后，运用农杆菌真空渗透法转化梨果，研究过表达关键GST基因对发病过程的影响，进一步验证其与虎皮病之间的关系。通过本项目的实施，将获得参与梨虎皮病发病的关键GST基因，探明其作用机制，为GST参与发病过程提供证据，同时也为今后培育具有抗虎皮病的梨优质种质资源提供理论指导。

Superficial scald, as a physiological disorder of pear fruit during storage and transportation, negatively affects their appearance quality, resulting in huge economic loss. Glutathione-S-transferase (GST), an antioxidant enzyme, is supposed to play an important role in the development of superficial scald through the participation in reactive oxygen species (ROS) metabolism, which is still unverified by experiment. In this study, ‘Dangshansuli’ pear is used as material. By comparing protein profiles in pear fruits with different grade of superficial scald using iTRAQ technology, a key GST gene which is closely relevant to superficial scald is selected after combining the results of bioinformatic analysis, Western blotting as well as intervention trail. After genetic transformation of tomato by the aid of over-expression vector pCAMBIA1301, the role of the key GST gene in ROS metabolism is further characterized by comparing the phenotypic difference between wildtype and transgenic fruits. Finally, the impact of overexpression of the key GST gene in pear pericarp on the development of superficial scald is studied with the aid of Agrobacterium-mediated vacuum infiltration to verify the relationship between the key GST gene and superficial scald. Our study is helpful to discover a key GST gene relevant to superficial scald and clarified its role, which will be benefit for providing the evidence for the participation of GST in superficial scald development and exploring superficial scald-resistant germplasm resource via providing the theoretical guidance.