Characterization of the glutathione S-transferase (GST) gene family in Pyrus bretschneideri and their expression pattern upon superficial scald development
Characterization of the glutathione S-transferase (GST) gene family in Pyrus bretschneideri and their expression pattern upon superficial scald development
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梨谷胱甘肽 S-转移酶 (GST) 基因家族的特征及其在浅烫伤中的表达模式
DOI：
10.1007/s10725-018-0422-4
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发表时间：
2018-06
影响因子：
4.2
通讯作者：
Shaoling Zhang
中科院分区：
文献类型：
--
作者：
Libin Wang;Ming Qian;Runze Wang;Li Wang;Shaoling Zhang
本研究共鉴定出62个GST家族基因，分布于14条染色体和8个支架上，且分布不均匀。系统发育分析表明，它们可分为8类，WGD/片段重复是它们扩展的主要驱动力。PbrGSTs在鸭梨果实中的表达具有组织特异性。鸭梨果皮中活性氧（ROS）的积累和抗坏血酸（阿萨）的损失伴随着果皮表层萎蔫的发生。在果皮中检测到的47个PbrGSTs中，25个成员的转录增强后的发展表浅的皱纹，而三个脱氢抗坏血酸还原酶（达尔）类下调与较低的达尔活性。结合1-MCP和MHO熏蒸对PbrGSTs表达谱影响的结果，筛选出PbrDHAR 1、PbrDHAR 2和PbrDHAR 4为参与浅表淋巴结发育的候选基因。
In this study, a total of 62 GST family genes were identified in Pyrus bretschneideri genome, which were distributed across the 14 chromosomes and 8 scaffolds with an uneven distribution. They could be categorized into eight classes based on phylogenetic analysis, and WGD/segmental duplication mainly drove their expansion. The expression of PbrGSTs in ‘Yali’ pear fruit was tissue-specific. In accompany with superficial scald development was the accumulation of reactive oxygen species (ROS) accumulation and loss of ascorbic acid (AsA) in the pericarp of ‘Yali’ pear. Of 47 PbrGSTs detected in the pericarp, the transcription of 25 members were enhanced upon the development of superficial scald, while three in dehydroascorbate reductase (DHAR) class were downregulated in association with lower DHAR activity. In combination with the results of the impact of 1-MCP and MHO fumigation on the expression profile of PbrGSTs, PbrDHAR1, PbrDHAR2 and PbrDHAR4 were selected as the candidate gene involved in superficial scald development.
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