骨髓间充质干细胞（BMSCs）良好的迁移性及肿瘤趋向性，成为结肠癌生物治疗的首选载体，但结肠癌微环境中，BMSCs可能发生恶性转化，其应用的安全性备受关注，能否找到一种抑制“恶变”、维持“干性”的方法，迄今并不清楚。藏药湿生扁蕾具有利湿、清热、解毒之功效，切合结肠癌湿热蕴结中医病机。我们前期研究发现，藏药湿生扁蕾可调控结肠癌相关基因CyclinD1等的表达、诱导人结肠癌SW480细胞凋亡，可调控致癌信号分子IL-6的表达、抑制IL-6/STAT3信号通路活化。我们推测藏药湿生扁蕾通过“调和”结肠癌微环境，可能有防止BMSCs恶变的作用。为证实该假说，我们拟建立大鼠体外BMSCs与SW480细胞共培养体系及裸鼠体内实体瘤模型，观察BMSCs恶变及体内成瘤情况，探讨藏药湿生扁蕾对结肠癌微环境中BMSCs恶性转化及体内成瘤的抑制作用及其机制，为BMSCs应用的安全性研究提供中医药的思路与方法。

Because of the favourable transfer and tumor tropism, BMSCs became the first choice carrier on biotherapy of colon carcinoma.But in colon carcinoma microenvironment, BMSCs malignant transformation possiblely happen, so a lot of attention were paid to the security of the application of BMSCs. It,s uncertain if we can find a method which could inhibit canceration and retain dry nature. Gentianopsis paludosa(Munro)Ma has the effect of clearing heat-toxin , eliminating dampness and detoxication, which consistent with the pathogenesis of colon carcinoma in chinese medical science. In previous study ,we found that, Gentianopsis paludosa(Munro)Ma could regulate the expression of colon carcinoma associated gene CyclinD1, induce apoptosis of human colon carcinoma cell SW480, regulate the expression of carcinogenesis signaling molecule IL-6, suppress the signal channel of IL-6/STAT3. We presume Gentianopsis paludosa(Munro)Ma have the effect of prevent BMSCs canceration by harmonize colon carcinoma microenvironment. In order to confirm this hypothesis, we plan to establish the synthem of rat BMSCs and SW480 co-culture in vitro and establish solid tumor model in nude mouse, observe canceration of BMSCs and state of tumor growth in vivo, investigate the inhibitory effect and the mechanism of Gentianopsis paludosa(Munro)Ma on the malignant transformation of BMSCs and tumor growth in vivo in colon carcinoma microenvironment, to provide traditional Chinese medicine method to the safety research of BMSCs application.