死亡时间（PMI）推断是刑事案件侦破的关键环节，腐败尸体PMI推断一直是法医学难题。法医昆虫学在腐败尸体PMI推断方面应用前景较好，其中嗜尸性麻蝇具备独特的优越性，尤其在气候特点鲜明、种类繁多的湖南地区，其应用价值较高。然而，种类鉴定困难和缺乏发育资料阻碍了嗜尸性麻蝇在法医实践中的应用。前期研究初步建立了嗜尸性麻蝇基因库，并发现基于信息性单核苷酸多态性（SNP）基因座建立的单倍型类群，有应用于麻蝇鉴定的潜力。本研究拟在前期工作基础上，收集湖南各地区实际案例中人尸体上麻蝇，全面检测mtDNA序列中的信息性SNP,确定单倍型类型，应用焦磷酸测序技术建立快速、准确、经济的嗜尸性麻蝇种类鉴定方法；明确湖南地区实际检案中嗜尸性麻蝇种类，收集并分析优势种群的发育数据和单倍型数据，构建湖南地区嗜尸性麻蝇资料库，探索应用嗜尸性麻蝇进行PMI推断的程序和方法，为在实践中应用嗜尸性麻蝇进行PMI推断奠定基础。

Postmortem interval (PMI) plays an essential role in forensic practice. PMI estimation of putrescent corpses has always been the area of research focus. The value of forensic entomology for PMI in forensic investigation has been well demonstrated in many case studies, research articles and books by entomologists and forensic investigators. Flesh flies have been attracting attention increasingly worldwide, as their special life cycle and habits. Many sarcophagid species in Hunan province have the potential to be used as PMI indicator. In our previous studies, preliminary GenBank of sarcophagids in China have been set up, and informational single nucleotide polymorphism loci, which can be used for species identification have been found in the mitochondrial DNA sequences. Based on the previous studies, sarcophagids samples will be collected and the informational single nucleotide polymorphism of mitochondrial DNA will be detceted. Then a quickly, accurate, economic species identification method and database of forensically important flesh flies will be established. Research builds upon the basis principles for growth and development of sarcophagid species will provide a new thought and method for PMI estimation, leading to higher accuracy of estimating the PMI for forensic practice.