Analysis of dendritic cells in tumor-free and tumor-containing sentinel lymph nodes from patients with breast cancer.
Analysis of dendritic cells in tumor-free and tumor-containing sentinel lymph nodes from patients with breast cancer.
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DOI：
10.1186/bcr808
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Grimm EA
中科院分区：
文献类型：
--
作者：
Poindexter NJ;Sahin A;Hunt KK;Grimm EA
Sentinel lymph node (SLN) biopsy allows identification of the first lymph node into which a primary tumor drains. In breast cancer, identification of tumor cells in the SLNs is a predictor of the tumor's metastatic potential. In the present article, we tested the hypotheses that a positive immune response can occur in tumor-free SLNs and that the activation state of dendritic cells (DCs), the major antigen presenting cells within SLNs, predicts the immune status and metastatic potential of the tumor. Fifty paraffin-embedded SLN sections, 25 tumor-free and 25 tumor-containing, from patients with breast cancer were analyzed by immunohistochemistry to determine the immune maturation state of their DCs. In addition, 12 lymph nodes from noncancer-containing breasts were analyzed. Tissues were stained with antibodies against CD3, MHC class II, CD1a, CD83, IL-10, and IL-12. Mature DCs were defined by CD83 expression and immature DCs by CD1a expression. We found a trend toward higher numbers of mature CD83-positive DCs in tumor-free SLNs than in tumor-containing SLNs (P = 0.07). In addition, tumor-free SLNs were more likely to contain cells expressing IL-10 (P = 0.02) and, to a lesser extent, IL-12 (P = 0.12). In contrast, when all SLNs, both tumor-free and tumor-containing, were compared with uninvolved lymph nodes, the numbers of mature and immature DCs were similar. Our results suggest tumor-free SLNs are immunologically competent and potentially a site of tumor-specific T-cell activation, as evidenced by the presence of greater numbers of mature DCs and cytokine-producing cells in tumor-free SLNs.
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DOI：
10.1073/pnas.93.6.2588
发表时间：
1996-03-19
影响因子：
11.1
作者：
Zhou, LJ;Tedder, TF
通讯作者：
Tedder, TF
影响因子：
5.4
作者：
Heufler, C;Koch, F;Schuler, G
通讯作者：
Schuler, G
影响因子：
45.3
作者：
Giuliano, AE;Jones, RC;Statman, R
通讯作者：
Statman, R
影响因子：
3.7
作者：
Leong, SPL
通讯作者：
Leong, SPL
DOI：
10.1084/jem.190.10.1417
发表时间：
1999-11-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bell D;Chomarat P;Broyles D;Netto G;Harb GM;Lebecque S;Valladeau J;Davoust J;Palucka KA;Banchereau J
通讯作者：
Banchereau J